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Applied Biochemistry and Biotechnology

, Volume 186, Issue 3, pp 576–589 | Cite as

Transesterification of Waste Frying Oil and Soybean Oil by Combi-lipases Under Ultrasound-Assisted Reactions

  • Jakeline Kathiele Poppe
  • Carla Roberta Matte
  • Roberto Fernandez-Lafuente
  • Rafael C. Rodrigues
  • Marco Antônio Záchia Ayub
Article

Abstract

This work describes the use of an ultrasound system for the enzymatic transesterification of oils using combi-lipases as biocatalyst. The reactions were carried out evaluating the individual use of waste oil and fresh soybean oil, and the immobilized lipases CALB, TLL, and RML were used as biocatalysts. It was performed in a mixture design of three factors to obtain the ideal mixture of lipases according to the composition of fatty acids present in each oil, and the main reaction variables were optimized. After 18 h of reaction, ultrasound provided a biodiesel yield of about 90% when using soybean oil and 70% using the waste oil. The results showed that ultrasound technology, in combination with the application of enzyme mixtures, known as combi-lipases, and the use of waste oil, could be a promising route to reduce the overall process costs of enzymatic production of biodiesel.

Keywords

Transesterification reaction Biodiesel Waste oil Soybean oil Combi-lipase Ultrasound system 

Notes

Acknowledgements

The authors would like to thank Mr. Ramiro Martinez (Novozymes, Spain S.A.) for kindly supplying the enzymes used in this research.

Funding Information

This work was supported by grants from the Brazilian Coordenação de Aperfoiçoamento de Pessoal de Nível Superior (CAPES) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflicts of interest.

Supplementary material

12010_2018_2763_MOESM1_ESM.docx (31 kb)
ESM 1 (DOCX 31 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Jakeline Kathiele Poppe
    • 1
  • Carla Roberta Matte
    • 1
  • Roberto Fernandez-Lafuente
    • 2
  • Rafael C. Rodrigues
    • 1
  • Marco Antônio Záchia Ayub
    • 1
  1. 1.Biotechnology, Bioprocess, and Biocatalysis Group, Food Science and Technology InstituteFederal University of Rio Grande do SulPorto AlegreBrazil
  2. 2.Department of BiocatalysisICP-CSIC, Campus UAM-CSICMadridSpain

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