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Applied Biochemistry and Biotechnology

, Volume 186, Issue 3, pp 535–546 | Cite as

Isolation and Structural Characterization of a Second Polysaccharide from Bulbs of Lanzhou Lily

  • Fengxia Wang
  • Wei Wang
  • Xiaobo Niu
  • Yulong Huang
  • Ji Zhang
Article
  • 182 Downloads

Abstract

In this study, a second water-soluble polysaccharide (designated as LDP-2) was isolated from the bulbs of Lanzhou Lily (Lilium davidii var. unicolor). Based on monosaccharide composition and methylation analysis, its structural features were investigated using a variety of characterizations, such as size-exclusion chromatography combined with laser light scattering (SEC-LLS), gas chromatography-mass spectroscopy (GC-MS), infrared (IR) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy. The results demonstrate that LDP-2 is a homogeneous heteropolysaccharide (molecular weight 6.2 × 104 Da), which includes four kinds of monosaccharides (Lyx, Man, Glc, and Gal in an approximate weight ratio of 6.74: 6.28: 76.50: 10.48). Furthermore, its morphology, thermal behavior, and preliminary hypoglycemic activities were also investigated.

Keywords

Polysaccharide Lanzhou Lily Purification Hypoglycemic activity 

Notes

Funding Information

We thank the National Natural Science Foundation of China (21561019) and Young Teachers Research Capacity Promotion Program of Northwest Normal University (NWNU-LKQN-16-19) as well as the Foundation of A Hundred Youth Talents Training Program of Lanzhou Jiaotong University.

Compliance with Ethical Standards

Conflicts of Interest

The authors declare that they have no conflict of interest.

Supplementary material

12010_2018_2750_MOESM1_ESM.doc (126 kb)
Fig. S1 (DOC 126 kb)

References

  1. 1.
    Munafo Jr. J., & Gianfagna, T. J. (2015). Quantitative analysis of phenylpropanoid glycerol glucosides in different organs of easter lily (Lilium longiflorum Thunb.). Journal of Agricultural & Food Chemistry, 63(19), 4836–4842.CrossRefGoogle Scholar
  2. 2.
    Jin, L., Zhang, Y., Yan, L., Guo, Y., & Niu, L. (2012). Phenolic compounds and antioxidant activity of bulb extracts of six Lilium species native to China. Molecules, 17(8), 9361–9378.CrossRefGoogle Scholar
  3. 3.
    Xu, L. F., Ma, F. W., & Dong, L. (2009). Plant regeneration from in vitro cultured leaves of Lanzhou Lily (Lilium davidii var. unicolor). Scientia Horticulturae, 119, 458–461.CrossRefGoogle Scholar
  4. 4.
    Zhang, J., Gao, Y., Zhou, X., Hu, L., & Xie, T. (2010). Chemical characterisation of polysaccharides from Lilium davidii. Natural Product Research, 24(4), 357–369.CrossRefGoogle Scholar
  5. 5.
    You, X. J., Xie, C. Y., Liu, K. L., & Gu, Z. X. (2010). Isolation of non-starch polysaccharides from bulb of tiger lily (Lilium lancifolium Thunb.) with fermentation of Saccharomyces cerevisiae. Carbohydrate Polymers, 81(1), 35–40.CrossRefGoogle Scholar
  6. 6.
    Zhang, D. N., Guo, X. Y., & Chen, Z. G. (2014). A novel and efficient method for the isolation and purification of polysaccharides from lily bulbs by Saccharomyces cerevisiae fermentation. Process Biochemistry, 49(12), 2299–2304.CrossRefGoogle Scholar
  7. 7.
    Kozarski, M., Klaus, A., Nikšić, M., Vrvić, M. M., Todorović, N., Jakovljević, D., & Van Griensven, L. J. L. D. (2012). Antioxidative activities and chemical characterization of polysaccharide extracts from the widely used mushrooms Ganoderma applanatum, Ganoderma lucidum, Lentinus edodes and Trametes versicolor. Journal of Food Composition and Analysis, 26(1-2), 144–153.CrossRefGoogle Scholar
  8. 8.
    Kozarski, M., Klaus, A., Jakovljevic, D., Todorovic, N., Niksic, M., Vrvic, M. M., & Griensven, L. J. L. D. V. (2014). Dietary polysaccharide extracts of Agaricus brasiliensis fruiting bodies: chemical characterization and bioactivities at different levels of purification. Food Research International, 64, 53–64.CrossRefGoogle Scholar
  9. 9.
    Chen, Z. G., Zhang, D. N., Zhu, Q., Yang, Q. H., & Han, Y. B. (2014). Purification, preliminary characterization and in vitro immunomodulatory activity of tiger lily polysaccharide. Carbohydrate Polymers, 106(1), 217–222.CrossRefGoogle Scholar
  10. 10.
    Yang, S., Li, Y., Jia, D., Yao, K., & Liu, W. (2017). The synergy of Box-Behnken designs on the optimization of polysaccharide extraction from mulberry leaves. Industrial Crops and Products, 99, 70–78.CrossRefGoogle Scholar
  11. 11.
    Angeli, J. P. F., Ribeiro, L. R., Camelini, C. M., de Mendonça, M. M., & Mantovani, M. S. (2009). Evaluation of the antigenotoxicity of polysaccharides and β-glucans from Agaricus blazei, a model study with the single cell gel electrophoresis/Hep G2 assay. Journal of Food Composition and Analysis, 22(7-8), 699–703.CrossRefGoogle Scholar
  12. 12.
    Moreira, M. R., Cassani, L., Martín-Belloso, O., & Soliva-Fortuny, R. (2015). Effects of polysaccharide-based edible coatings enriched with dietary fiber on quality attributes of fresh-cut apples. Journal of Food Science & Technology, 52(12), 7795–7805.CrossRefGoogle Scholar
  13. 13.
    Ren, L., Edwards, P. J. B., Perera, C. O., & Hemar, Y. (2015). Structural features of a novel polysaccharide isolated from a New Zealand Maori mushroom Iliodiction cibarium. Carbohydrate Research, 406(10), 19–26.CrossRefGoogle Scholar
  14. 14.
    Zhao, B., Zhang, J., Guo, X., & Wang, J. (2013). Microwave-assisted extraction, chemical characterization of polysaccharides from Lilium davidii var. unicolor Salisb and its antioxidant activities evaluation. Food Hydrocolloids, 31(2), 346–356.CrossRefGoogle Scholar
  15. 15.
    Wang, J. H., Du, Y. Q., Sun, H. J., & Zhang, J. C. (2015). Extraction and preliminary characterization of polysaccharide from Umbilicaria esculenta cultivated in Huangshan Mountain. Biotechnology & Biotechnological Equipment, 29(4), 714–722.CrossRefGoogle Scholar
  16. 16.
    Liu, Y., Liu, C., Jiang, H., Zhou, H., Li, P., & Wang, F. (2015). Isolation, structural characterization and neurotrophic activity of a polysaccharide from Phellinus ribis. Carbohydrate Polymers, 127(5), 145–151.CrossRefGoogle Scholar
  17. 17.
    Wang, F., Wang, W., Huang, Y., Liu, Z., & Zhang, J. (2015). Characterization of a novel polysaccharide purified from a herb of Cynomorium songaricum Rupr. Food Hydrocolloids, 47, 79–86.CrossRefGoogle Scholar
  18. 18.
    Wang, Z. J., Luo, D. H., & Liang, Z. Y. (2004). Structure of polysaccharides from the fruiting body of Hericium erinaceus Pers. Carbohydrate Polymers, 57(3), 241–247.CrossRefGoogle Scholar
  19. 19.
    Gu, J. F., Zheng, Z. Y., Yuan, J. R., Zhao, B. J., Wang, C. F., Zhang, L., & Jia, X. B. (2015). Comparison on hypoglycemic and antioxidant activities of the fresh and dried Portulaca oleracea L. in insulin-resistant HepG2 cells and streptozotocin-induced C57BL/6J diabetic mice. Journal of Ethnopharmacology, 161(23), 214–223.CrossRefGoogle Scholar
  20. 20.
    Zhang, H. J., Ji, B. P., Chen, G., Zhou, F., Luo, Y. C., Yu, H. Q., & Li, H. Y. (2009). A combination of grape seed-derived procyanidins and gypenosides alleviates insulin resistance in mice and HepG2 cells. Journal of Food Science, 74(1), H1–H7.CrossRefGoogle Scholar
  21. 21.
    Li, Y., Sun, L., Jin, M., Du, Z., Liu, X., Guo, C., & Sun, Z. (2011). Size-dependent cytotoxicity of amorphous silica nanoparticles in human hepatoma HepG2 cells. Toxicology In Vitro, 25(7), 1343–1352.CrossRefGoogle Scholar
  22. 22.
    Yin, J., Hu, R., Chen, M., Tang, J., Li, F., Yang, Y., & Chen, J. (2002). Effects of berberine on glucose metabolism in vitro. Metabolism Clinical & Experimental, 51(11), 1439–1443.CrossRefGoogle Scholar
  23. 23.
    Chen, H. X., Zhang, M., Qu, Z. H., & Xie, B. J. (2008). Antioxidant activities of different fractions of polysaccharide conjugates from green tea (Camellia Sinensis). Food Chemistry, 106(2), 559–563.CrossRefGoogle Scholar
  24. 24.
    Cui, J., Gu, X., Wang, F., Ouyang, J., & Wang, J. (2015). Purification and structural characterization of an α-glucosidase inhibitory polysaccharide from apricot (Armeniaca sibirica L. Lam.) pulp. Carbohydrate Polymers, 121(5), 309–314.CrossRefGoogle Scholar
  25. 25.
    Zheng, C., Dong, Q., Du, Z., Wang, P., & Ding, K. (2015). Structural elucidation of a polysaccharide from Chrysanthemum morifolium flowers with anti-angiogenic activity. International Journal of Biological Macromolecules, 79, 674–680.CrossRefGoogle Scholar
  26. 26.
    Hu, H., Liang, H., & Wu, Y. (2015). Isolation, purification and structural characterization of polysaccharide from Acanthopanax brachypus. Carbohydrate Polymers, 127(20), 94–100.CrossRefGoogle Scholar
  27. 27.
    Sun, W., Meng, K., Qi, C., Yang, X., Wang, Y., Fan, W., & Liu, J. (2015). Immune-enhancing activity of polysaccharides isolated from Atractylodis macrocephalae Koidz. Carbohydrate Polymers, 126(1), 91–96.CrossRefGoogle Scholar
  28. 28.
    Mitić, Ž., Cakić, M., Nikolić, G. M., Nikolić, R., Nikolić, G. S., Pavlović, R., & Santaniello, E. (2011). Synthesis, physicochemical and spectroscopic characterization of copper(II)-polysaccharide pullulan complexes by UV–vis, ATR-FTIR, and EPR. Carbohydrate Research, 346(3), 434–441.CrossRefGoogle Scholar
  29. 29.
    Miao, M., Bai, A., Jiang, B., Yang, S., Cui, S. W., & Zhang, T. (2014). Characterisation of a novel water-soluble polysaccharide from Leuconostoc citreum SK24.002. Food Hydrocolloids, 36(5), 265–272.CrossRefGoogle Scholar
  30. 30.
    Wu, G. H., Hu, T., Huang, Z. L., & Jiang, J. G. (2013). Characterization of water and alkali-soluble polysaccharides from Pleurotus tuber-regium sclerotia. Carbohydrate Polymers, 96(1), 284–290.CrossRefGoogle Scholar
  31. 31.
    Li, J., Fan, L., & Ding, S. (2011). Isolation, purification and structure of a new water-soluble polysaccharide from Zizyphus jujuba cv. Jinsixiaozao. Carbohydrate Polymers, 83(2), 477–482.CrossRefGoogle Scholar
  32. 32.
    Parikh, A., & Madamwar, D. (2006). Partial characterization of extracellular polysaccharides from cyanobacteria. Bioresource Technology, 97(15), 1822–1827.CrossRefGoogle Scholar
  33. 33.
    Nguyen, T. A., Do, T. T., Nguyen, T. D., Le, D. P., & Nguyen, V. D. (2011). Isolation and characteristics of polysaccharide from Amorphophallus corrugatus in Vietnam. Carbohydrate Polymers, 84(1), 64–68.CrossRefGoogle Scholar
  34. 34.
    Zakula, Z., Koricanac, G., Tepavcevic, S., Stojiljkovic, M., Milosavljevic, T., & Isenovic, E. R. (2011). Impairment of cardiac insulin signaling in fructose-fed ovariectomized female Wistar rats. European Journal of Nutrition, 50(7), 543–551.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.College of Life ScienceNorthwest Normal UniversityLanzhouChina
  2. 2.Bioactive Products Engineering Research Center for Gansu Distinctive PlantsLanzhouChina
  3. 3.School of Chemical and Biological EngineeringLanzhou Jiaotong UniversityLanzhouChina

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