Effect of Enzymatic Digestion of Protein Derivatives Obtained from Mucuna pruriens L. on Production of Proinflammatory Mediators by BALB/c Mouse Macrophages
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Inflammation is considered to be a major risk factor for the pathogenesis of chronic non-communicable diseases. Macrophages are important immune cells, which regulate inflammation and host defense by secretion of proinflammatory mediators. Obtaining biopeptides by enzymatic hydrolysis adds value to proteins of vegetative origin, such as Mucuna pruriens L. The present study evaluated the effect of enzymatic digestion of protein derivatives obtained from M. pruriens L. on the production of proinflammatory mediators by BALB/c mouse macrophages. Five different molecular weight peptide fractions were obtained (F > 10, 5–10, 3–5, 1–3, and < 1 kDa, respectively). At 300 μg/mL, F5–10 kDa inhibited 50.26 and 61.00% NO and H2O2 production, respectively. Moreover, F5–10 kDa reduced the IL-6 and TNFα levels to 60.25 and 69.54%, respectively. After enzymatic digestive simulation, F5–10 kDa decreased the inflammatory mediators.
KeywordsBioactive compounds Anti-inflammatory activity Protein hydrolyzate Inflammation
This paper is part of the “Scientific Research Development Directed to develop Protein Derivative of Mucuna pruriens with Potential Biological Activity for Prevention and / or Treatment of Chronic Diseases Associated with Overweight and Obesity” project funded by CONACYT-Mexico (Project 154307).
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Conflict of Interest
The authors declare they have no conflicts of interest.
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