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Applied Biochemistry and Biotechnology

, Volume 186, Issue 3, pp 633–643 | Cite as

A PDMS Device Coupled with Culture Dish for In Vitro Cell Migration Assay

  • Xiaoqing Lv
  • Zhaoxin Geng
  • Zhiyuan Fan
  • Shicai Wang
  • WeiHua Pei
  • Hongda Chen
Article

Abstract

Cell migration and invasion are important factors during tumor progression and metastasis. Wound-healing assay and the Boyden chamber assay are efficient tools to investigate tumor development because both of them could be applied to measure cell migration rate. Therefore, a simple and integrated polydimethylsiloxane (PDMS) device was developed for cell migration assay, which could perform quantitative evaluation of cell migration behaviors, especially for the wound-healing assay. The integrated device was composed of three units, which included cell culture dish, PDMS chamber, and wound generation mold. The PDMS chamber was integrated with cell culture chamber and could perform six experiments under different conditions of stimuli simultaneously. To verify the function of this device, it was utilized to explore the tumor cell migration behaviors under different concentrations of fetal bovine serum (FBS) and transforming growth factor (TGF-β) at different time points. This device has the unique capability to create the “wound” area in parallel during cell migration assay and provides a simple and efficient platform for investigating cell migration assay in biomedical application.

Keywords

Polydimethylsiloxane (PDMS) Cell migration In vitro Transforming growth factor-β 

Notes

Funding Information

The project was supported by Strategic Priority Research Program of the Chinese Academy of Sciences (XDA16020900), the National Key Research and Development Plan (2016YFB0402700 and 2017YFB0405400), the National Natural Science Foundation of China (61774175, 61674146, 61378058, 61335010, and 11574408), the Opened Fund of the State Key Laboratory of Integrated Optoelectronics (No.IOSKL2017KF12), and The Key Program of Natural Science Foundation of Beijing (4181001)t

Compliance with Ethical Standards

Conflict of Interest

The authors declare no competing interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Xiaoqing Lv
    • 1
    • 2
  • Zhaoxin Geng
    • 1
    • 3
  • Zhiyuan Fan
    • 1
    • 2
  • Shicai Wang
    • 4
  • WeiHua Pei
    • 1
  • Hongda Chen
    • 1
  1. 1.State Key Laboratory of Integrated Optoelectronics, Institute of SemiconductorsChinese Academy of SciencesBeijingChina
  2. 2.College of Materials Science and Opto-Electronic TechnologyUniversity of Chinese Academy of SciencesBeijingChina
  3. 3.School of Information EngineeringMinzu University of ChinaBeijingChina
  4. 4.State Key Laboratory of crystal materialsShandong UniversityJinanChina

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