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Applied Biochemistry and Biotechnology

, Volume 186, Issue 1, pp 233–244 | Cite as

Isolation and Characterization of PHA-Producing Bacteria from Propylene Oxide Saponification Wastewater Residual Sludge

  • Ruirui Li
  • Pengfei Gu
  • Xiangyu Fan
  • Junyu Shen
  • Yulian Wu
  • Lixuan Huang
  • Qiang Li
Article
  • 256 Downloads

Abstract

A polyhydroxyalkanoate (PHA)-producing strain was isolated from propylene oxide (PO) saponification wastewater activated sludge and was identified as Brevundimonas vesicularis UJN1 through 16S rDNA sequencing and Biolog microbiological identification. Single-factor and response surface methodology experiments were used to optimize the culture medium and conditions. The optimal C/N ratio was 100/1.04, and the optimal carbon and nitrogen sources were sucrose (10 g/L) and NH4Cl (0.104 g/L) respectively. The optimal culture conditions consisted of initial pH of 6.7 and an incubation temperature of 33.4 °C for 48 h, with 15% inoculum and 100 mL medium at an agitation rate of 180 rpm. The PHA concentration reached 34.1% of the cell dry weight and increased three times compared with that before optimization. The only report of PHA-producing bacteria by Brevundimonas vesicularis showed that the conversion rate of PHAs using glucose as the optimal carbon source was 1.67%. In our research, the conversion rate of PHAs with sucrose as the optimal carbon source was 3.05%, and PHA production using sucrose as the carbon source was much cheaper than that using glucose as the carbon source.

Keywords

Polyhydroxyalkanoates Brevundimonas vesicularis Optimization 

Notes

Funding Information

This work was supported by the National Natural Science Foundations of China (31741007, 31600066, 31600148), the Shandong Provincial Natural Science Foundations (ZR2016CL02, ZR2016CB20), and the Shandong Province Science and Technology Development Plan (2013GSF12006).

Compliance with Ethical Standards

Conflict of Interest

The authors declared that they have no conflicts of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.School of Biological Science and TechnologyUniversity of JinanJinanChina

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