Direct Utilization of Non-pretreated Hydrolytic Liquid of Dried Distiller’s Grains with Solubles for Bio-Ethanol by Rhizopus arrhizus RH 7-13-9#
- 93 Downloads
Bio-ethanol, as an environment friendly and renewable fuel, has gained increasing worldwide attention and can be produced through the fermentation of the carbohydrates or sugar(s) fraction of biomass materials. Here, dried distiller’s grains with solubles (DDGS), a waste in production of bio-ethanol, were applied to prepare acid-hydrolytic liquid and it was directly used as seed culture and fermentation medium without pretreatment. Rhizopus arrhizus RH 7-13-9# cultured in non-pretreated acid-hydrolytic liquid with pH 4.7 for 30 h could utilize the concentrated hydrolytic liquid as well as the hydrolytic liquid mixed with glucose. A high yield of bio-ethanol was obtained. It was proven that common used medium could be replaced by non-pretreated acid-hydrolytic liquid to some extent. The pretreated process of acid-hydrolytic liquid was avoided that decreased feed stock cost and was significant for the utilization of acid-hydrolytic liquid from lignocellulose materials.
KeywordsBio-ethanol Dried distiller’s grains with solubles (DDGS) Fermentation Rhizopus arrhizus
This research was financially supported by National Key research program (2016YFD0400601, 2017YFD0400603, 2017YFB0306900), the Natural Science Foundation of China (21476017), the Hong Kong, Macao, and Taiwan scientific and technological cooperation projects (2015DFT30050), the Amoy Industrial Biotechnology R&D and Pilot Conversion Platform (3502Z20121009), and the Fundamental Research Funds for the Central Universities (PYBZ1712).
Compliance with Ethical Standards
Conflicts of Interest
The authors declared that they have no conflicts of interest.
- 10.Huang, Y., Qin, X., Luo, X.-M., Nong, Q., Yang, Q., Zhang, Z., Gao, Y., Lv, F., Chen, Y., Yu, Z., Liu, J.-L., & Feng, J.-X. (2015). Efficient enzymatic hydrolysis and simultaneous saccharification and fermentation of sugarcane bagasse pulp for ethanol production by cellulase from Penicillium oxalicum EU2106 and thermotolerant Saccharomyces cerevisiae ZM1-5. Biomass and Bioenergy, 77, 53–63.CrossRefGoogle Scholar
- 14.Gao, Z., Zhang, K., Huang, H., Li, S., & Wei, P. (2009). Fumaric acid production by Rhizopus sp. Progress in Chemistry, 251–258.Google Scholar
- 15.Liu, H., Ma, J., Wang, M., Wang, W., Deng, L., Nie, K., Yue, X., Wang, F., & Tan, T. (2016). Food waste fermentation to fumaric acid by Rhizopus arrhizus RH7-13. Applied Biochemistry and Biotechnology, 1–10.Google Scholar