Effect of Different Carbon Sources on Cellulase Production by Marine Strain Microbulbifer hydrolyticus IRE-31-192

  • Huan Liu
  • Liping Zeng
  • Yuhan Jin
  • Kaili Nie
  • Li DengEmail author
  • Fang Wang


Cellulase is an important enzyme that can be used to breakdown lignocellulose into glucose. Microbulbifer hydrolyticus IRE-31(ATCC 700072) is a kind of marine bacterium, which could grow in high salinity medium and has fast-strong growth ability. In this study, a novel strain was screened from Microbulbifer hydrolyticus IRE-31 through mutations to produce cellulase. The effect of different carbon sources on the growth as well as on the production of cellulase of the new strain was studied. Carboxymethyl-cellulase (CMCase) activity selected to represent cellulase was proven to be effectively promoted while xylose, galactose, and melibiose as well as glucose were used as carbon sources. When xylose and glucose were chosen to be further investigated, 472.57 U/L and 266.01 U/L CMCase activity were obtained from 30 g/L glucose and 10 g/L xylose, respectively. These results clarified the effect of different carbon sources on the production of cellulase, which laid a good foundation for the further research in the production of cellulase by marine bacteria.


Marine bacteria Cellulase CMCase Fermentation 


Funding Information

This research was financially supported by the National Key Research Program (2016YFD0400601, 2017YFD0400603, 2017YFB0306900), the Natural Science Foundation of China (21476017), the Hong Kong, Macao, and Taiwan Scientific And Technological Cooperation Projects (2015DFT30050), and the Amoy Industrial Biotechnology R&D and Pilot Conversion Platform (3502Z20121009).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.


  1. 1.
    Neelamegam Annamalaia, ,, Rajeswarib, M. V. and Balasubramanianb, T. (2014) Enzymatic saccharification of pretreated rice straw by cellulase produced from Bacillus carboniphilus CAS 3 utilizing lignocellulosic wastes through statistical optimization. Biomass and Bioenergy, 151–160, 68.CrossRefGoogle Scholar
  2. 2.
    Rambo, M. K., Schmidt, F. L., & Ferreira, M. M. (2015). Analysis of the lignocellulosic components of biomass residues for biorefinery opportunities. Talanta, 144, 696–703.CrossRefGoogle Scholar
  3. 3.
    Román-Leshkov, Y., Barrett, C. J., Liu, Z. Y., & Dumesic, J. A. (2007). Production of dimethylfuran for liquid fuels from biomass-derived carbohydrates. NATURE, 447(7147), 982–985.CrossRefGoogle Scholar
  4. 4.
    Wang, K., Kim, K. H., & Brown, R. C. (2014). Catalytic pyrolysis of individual components of lignocellulosic biomass. Green Chemistry, 16(2), 727–735.CrossRefGoogle Scholar
  5. 5.
    Mohan, M., Balaji, C., Goud, V. V., & Banerjee, T. (2015). Thermodynamic insights in the separation of cellulose/hemicellulose components from lignocellulosic biomass using ionic liquids. Journal of Solution Chemistry, 44(3-4), 538–557.CrossRefGoogle Scholar
  6. 6.
    Liu, H., Wang, W., Deng, L., Wang, F., & Tan, T. (2015). High production of fumaric acid from xylose by newly selected strain Rhizopus arrhizus RH 7-13-9#. Bioresource Technology, 186, 348–350.CrossRefGoogle Scholar
  7. 7.
    Kataoka, N., Vangnai, A. S., Ueda, H., Tajima, T., Nakashimada, Y., & Kato, J. (2014). Enhancement of (R)-1,3-butanediol production by engineered Escherichia coli using a bioreactor system with strict regulation of overall oxygen transfer coefficient and pH. Bioscience Biotechnology and Biochemistry, 78(4), 695–700.CrossRefGoogle Scholar
  8. 8.
    Srivastava, N., Rawat, R., Singh Oberoi, H., & Ramteke, P. W. (2014). A review on fuel ethanol production from lignocellulosic biomass. International Journal of Green Energy, 12, 949–960.CrossRefGoogle Scholar
  9. 9.
    Chen, M., Zhao, J., & Xia, L. x. z. e. c. (2009). Comparison of four different chemical pretreatments of corn stover for enhancing enzymatic digestibility. Biomass and Bioenergy, 33(10), 1381–1385.CrossRefGoogle Scholar
  10. 10.
    Singhania, R. R., Sukumaran, R. K., Patel, A. K., Larroche, C., & Pandey, A. (2010). Advancement and comparative profiles in the production technologies using solid-state and submerged fermentation for microbial cellulases. Enzyme Microb Tech, 46(7), 541–549.CrossRefGoogle Scholar
  11. 11.
    Maki, M. L., Broere, M., Leung, K. T., & Qin, W. (2011). Characterization of some efficient cellulase producing bacteria isolated from paper mill sludges and organic fertilizers. International Journal of Biochemistry and Molecular Biology, 146–154.Google Scholar
  12. 12.
    Yan, T. R., Lin, Y. H., & Lin, C. L. (1998). Purification and characterization of an extracellular beta-glucosidase II with high hydrolysis and transglucosylation activities from Aspergillus niger. Raven Press.Google Scholar
  13. 13.
    Bansal, N., Janveja, C., Tewari, R., Soni, R., & Soni, S. K. (2014). Highly thermostable and pH-stable cellulases from Aspergillus niger NS-2: properties and application for cellulose hydrolysis. Applied Biochemistry and Biotechnology, 172(1), 141–156.CrossRefGoogle Scholar
  14. 14.
    Dashtban, M., Buchkowski, R., & Qin, W. (2011). Effect of different carbon sources on cellulase production by Hypocrea jecorina (Trichoderma reesei) strains. International Journal of Biochemistry and Molecular Biology, 274–286.Google Scholar
  15. 15.
    Shi, Q.-Q., Sun, J., Yu, H.-L., Li, C.-X., Bao, J., & Xu, J.-H. (2011). Catalytic performance of corn stover hydrolysis by a new isolate Penicillium sp. ECU0913 producing both cellulase and xylanase. Applied Biochemistry and Biotechnology, 819–830.Google Scholar
  16. 16.
    Marjamaa, K., Toth, K., Bromann, P. A., Szakacs, G., & Kruus, K. (2013). Novel Penicillium cellulases for total hydrolysis of lignocellulosics. Enzyme and Microbial Technology, 52(6-7), 358–369.CrossRefGoogle Scholar
  17. 17.
    YP, P., & Qin, W. (2015). Characterization of novel cellulase-producing bacteria isolated from rotting wood samples. Applied Biochemistry and Biotechnology, 1186–1198.Google Scholar
  18. 18.
    Wang, C.-M., Shyu, C.-L., Ho, S.-P., & Chiou, S.-H. (2008). Characterization of a novel thermophilic, cellulose-degrading bacterium Paenibacillus sp. strain B39. Letters in Applied Microbiology, 47(1), 46–53.CrossRefGoogle Scholar
  19. 19.
    Li, X., & Gao, P. (1997). Isolation and partial properties of cellulose-decomposing strain of Cytophaga sp. LX-7 from soil. Journal of Applied Microbiology, 82(1), 73–80.CrossRefGoogle Scholar
  20. 20.
    Rajoka, M. I., & Malik, K. A. (1997). Cellulase production by Cellulomonas biazotea cultured in media containing different cellulosic substrates. Bioresource Technology, 59(1), 21–27.CrossRefGoogle Scholar
  21. 21.
    González, J. M., Mayer, F., Moran, M. A., Hodson, R. E., & Whitman, W. B. (1997). Microbulbifer hydrolyticus gen. nov., sp. nov., and Marinobacterium georgiense gen. nov., sp. nov., two marine bacteria from a lignin-rich pulp mill waste enrichment community. International Journal of Systematic Bacteriology, 369–376.Google Scholar
  22. 22.
    Arens, K., & Liu, S. (2013). Induction of cellulase production by Microbulbifer hydrolyticus with hot-water wood extract. Journal of Bioprocess Engineering and Biorefinery, 262–270.Google Scholar
  23. 23.
    Samira, M., Mohammad, R., & Gholamreza, G. (2011). Carboxymethyl-cellulase and filter-paperase activity of new strains isolated from Persian Gulf. Microbiology Journal, 8–16.Google Scholar
  24. 24.
    Singh, S., Dikshit, P. K., Moholkar, V. S., & Goyal, A. (2015). Purification and characterization of acidic cellulase from Bacillus amyloliquefaciens SS35 for hydrolyzing Parthenium hysterophorusbiomass. Environmental Progress & Sustainable Energy, 34(3), 810–818.CrossRefGoogle Scholar
  25. 25.
    Hu, R., Lin, L., Liu, T., Ouyang, P., He, B., & Liu, S. (2008). Reducing sugar content in hemicellulose hydrolysate by DNS method: a revisit. Journal of Biobased Materials and Bioenergy, 2(2), 156–161.CrossRefGoogle Scholar
  26. 26.
    Miller, G. L. (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry, 31(3), 426–428.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Huan Liu
    • 1
  • Liping Zeng
    • 1
  • Yuhan Jin
    • 1
  • Kaili Nie
    • 1
    • 2
  • Li Deng
    • 1
    • 2
    Email author
  • Fang Wang
    • 1
  1. 1.Beijing Bioprocess Key Laboratory, State Key Laboratory of Chemical Resource EngineeringBeijing University of Chemical TechnologyBeijingPeople’s Republic of China
  2. 2.Amoy - BUCT Industrial Bio-technovation InstituteAmoyPeople’s Republic of China

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