Adsorption Study of Acid Soluble Lignin Removal from Sugarcane Bagasse Hydrolysate by a Self-Synthesized Resin for Lipid Production
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An adsorption resin CX-6 was synthesized and used for acid soluble lignin (ASL) removal from sugarcane bagasse hydrolysate (SCBH). The adsorption conditions of pH value, amount of adsorbent, initial ASL concentration, and temperature on ASL adsorption were discussed. The results showed the adsorption capacity of ASL was negatively affected by increasing temperature, solution pH, and adsorbent dose, and was positively affected by increasing initial concentration. The maximum adsorption capacity of ASL was 135.3 mg/g at initial ASL concentration 6.46 g/L, adsorption temperature 298 K, and pH 1. Thermodynamic study demonstrated that the adsorption process was spontaneous and exothermic. Equilibrium and kinetics experiments were proved to fit the Freundlich isotherm model and pseudo-second-order model well, respectively. Fermentation experiment showed that the SCBH after combined overliming with resin adsorption as fermentation substrate for microbial lipid production by Trichosporon cutaneum and Trichosporon coremiiforme was as better as that of SCBH by combined overliming with active charcoal adsorption, and more efficient than that of SCBH only by overliming. Moreover, the regeneration experiment indicated that the CX-6 resin is easy to regenerate and its recirculated performance is stable. In conclusion, our results provide a promising adsorbent to detoxify lignocellulose hydrolysate for further fermentation.
KeywordsAcid soluble lignin Sugarcane bagasse hydrolysate Adsorption resin Detoxification Microbial lipid production
This work was supported by Pearl River S&T Nova Program of Guangzhou (201610010014, 201710010096), the Project of National Natural Science Foundation of China (21606229, 51876207), the foundation of Key Laboratory of Renewable Energy, Chinese Academy of Sciences (Y707j41001), and the financial support of the Science and Technology Planning Project of Guangdong Province, China (2017A010103043).
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
- 7.Canilha, L., Chandel, A. K., Milessi, T. S. D., et al. (2012). Bioconversion of sugarcane biomass into ethanol: an overview about composition, pretreatment methods, detoxification of hydrolysates, enzymatic saccharification, and ethanol fermentation. Journal of Biomedicine and Biotechnology, 2012, 1–15. https://doi.org/10.1155/2012/989572.CrossRefGoogle Scholar
- 9.Kim, S. J., Kim, T. H., & Oh, K. K. (2018). Deacetylation followed by fractionation of yellow poplar sawdust for the production of toxicity-reduced hemicellulosic sugar for ethanol fermentation. Energies, 11(2). https://doi.org/10.3390/en11020404.
- 10.Sivagurunathan, P., Kumar, G., Mudhoo, A., Rene, E. R., Saratale, G. D., Kobayashi, T., Xu, K., Kim, S. H., & Kim, D. H. (2017). Fermentative hydrogen production using lignocellulose biomass: an overview of pre-treatment methods, inhibitor effects and detoxification experiences. Renewable and Sustainable Energy Reviews, 77, 28–42.CrossRefGoogle Scholar
- 19.Zhang, J. A., Zhu, Z. N., Wang, X. F., et al. (2010). Biodetoxification of toxins generated from lignocellulose pretreatment using a newly isolated fungus, Amorphotheca resinae ZN1, and the consequent ethanol fermentation. Biotechnology for Biofuels, 3(1), 26. https://doi.org/10.1186/1754-6834-3-26.CrossRefGoogle Scholar
- 21.Zhang, Y., Xia, C. L., Lu, M. M., & Tu, M. B. (2018). Effect of overliming and activated carbon detoxification on inhibitors removal and butanol fermentation of poplar prehydrolysates. Biotechnology for Biofuels, 11(1). https://doi.org/10.1186/s13068-018-1182-0.
- 25.Sluiter, A., Hames, B., Ruiz, R., Scarlata, C., Sluiter, J., Templeton, D., Crocker, D.. (2008). Determination of structural carbohydrates and lignin in biomass. National Renewable Energy Lab.Google Scholar
- 29.Lin, X. Q., Xiong, L., Huang, C., et al. (2016). Sorption behavior and mechanism investigation of formic acid removal by sorption using an anion-exchange resin. Desalination and Water Treatment, 57, 366–381.Google Scholar
- 31.Freundlich, H. (1906). Concerning adsorption in solutions. Zeitschrift Fur Physikalische Chemie--Stochiometrie Und Verwandtschaftslehre, 57, 385–470.Google Scholar
- 33.de Carvalho, W., Canilha, L., Mussatto, S. I., Dragone, G., Morales, M. L. V., & Solenzal, A. I. N. (2004). Detoxification of sugarcane bagasse hemicellulosic hydrolysate with ion-exchange resins for xylitol production by calcium alginate-entrapped cells. Journal of Chemical Technology and Biotechnology, 79(8), 863–868.CrossRefGoogle Scholar