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Applied Biochemistry and Biotechnology

, Volume 188, Issue 2, pp 491–497 | Cite as

Detection of Antibodies Against Foot-and-Mouth Disease Virus Serotypes A, O and Asia-1 by ELISA in Strip-Dried Samples from Vaccinated Bovines

  • Jeanne V. SamsonovaEmail author
  • Nikolay Yu. Saushkin
  • Alexander P. Osipov
  • Sergey E. Kondakov
  • Svetlana N. Fomina
  • Alexey V. Mischenko
Article
  • 98 Downloads

Abstract

Antibodies against foot-and-mouth disease virus serotypes A, O and Asia-1 were detected by ELISA in liquid and strip-dried samples from vaccinated bovines. The results showed high concordance of the results in both types of serum samples (coefficient of correlation varied from 0.89 to 0.96). Stability studies showed that anti-foot-and-mouth disease virus antibodies can be detected in strip-dried serum samples stored at different temperatures on a level of that in native samples. Preliminary study in strip-dried whole blood samples demonstrated good potential of this sample pretreatment procedure for the following anti-foot-and-mouth virus antibodies testing.

Keywords

Strip-dried samples Foot-and-mouth disease Bovine ELISA 

Notes

Funding Information

This work was financially supported by the Ministry of Education and Science of the Russian Federation as a based part of state assignment organization of scientific researches (project No. 16.6548.2017/BY).

Compliance with Ethical Standards

Conflict of Interest Statement

None of the authors of this paper has a financial or personal relationship with other people or organisations that could inappropriately influence or bias the content of the paper.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Faculty of ChemistryM.V. Lomonosov Moscow State UniversityMoscowRussia
  2. 2.National University of Science and Technology “MISiS”MoscowRussia
  3. 3.Federal Centre for Animal HealthVladimirRussia

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