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Applied Biochemistry and Biotechnology

, Volume 188, Issue 2, pp 450–459 | Cite as

Gibberellin Promotes Cell Growth and Induces Changes in Fatty Acid Biosynthesis and Upregulates Fatty Acid Biosynthetic Genes in Chlorella vulgaris UMT-M1

  • Malinna Jusoh
  • Saw Hong Loh
  • Ahmad Aziz
  • Thye San ChaEmail author
Article
  • 189 Downloads

Abstract

Microalgae lipids and oils are potential candidates for renewable biofuels and nutritional inventions. Recent studies from our lab have shown that two plant hormones, auxin and jasmonic acid, influence microalgae growth and fatty acid accumulation. Therefore, in this study, a high oil-producing strain Chlorella vulgaris UMT-M1 was selected for hormonal study using gibberellin (GA). Exogenous GA3 was applied to early stationary culture of C. vulgaris UMT-M1. Results showed that GA3 gradually increases the cell density of C. vulgaris to up to 42% on days after treatment (DAT)-8 and also capable of delaying the algal senescence. However, the increment in cell density did not enhance the total oil production albeit transient modification of fatty acid compositions was observed for saturated (SFA) and polyunsaturated (PUFA) fatty acids. This illustrates that GA3 only promotes cell division and growth but not the oil accumulation. In addition, application of GA3 in culture medium was shown to promote transient increment of palmitic (C16:0) and stearic (C18:0) acids from DAT-4 to DAT-6 and these changes are correlated with the expression of β-ketoacyl ACP synthase I (KAS I) gene.

Keywords

Fatty acid Gene expression Lipid Microalgae Plant hormone 

Notes

Funding Information

This project was financially supported by the Science Fund (Project No. 02-01-12-SF0089) from the Ministry of Science, Technology and Innovation (MOSTI), Malaysia.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Consent for Publication

All authors approved the manuscript.

Supplementary material

12010_2018_2937_MOESM1_ESM.docx (14 kb)
ESM 1 (DOCX 14 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Institute of Marine BiotechnologyUniversiti Malaysia TerengganuKuala TerengganuMalaysia
  2. 2.School of Fundamental SciencesUniversiti Malaysia TerengganuKuala TerengganuMalaysia
  3. 3.School of Marine and Environmental SciencesUniversiti Malaysia TerengganuKuala TerengganuMalaysia

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