Applied Biochemistry and Biotechnology

, Volume 188, Issue 2, pp 540–554 | Cite as

Isolation and Nitrogen Removal Characteristics of an Aerobic Heterotrophic Nitrifying-Denitrifying Bacterium, Klebsiella sp. TN-10

  • Dan Li
  • Xihong Liang
  • Yao Jin
  • Chongde WuEmail author
  • Rongqing Zhou


Nitrogen removal by microorganisms has attracted increasing attention in wastewater treatment. In the present study, a heterotrophic nitrification bacterium was isolated from tannery wastewater and identified as Klebsiella sp. TN-10 based on phenotypic and phylogenetic characteristics. The optimal conditions for cell growth and nitrogen removal were investigated, and the results showed that the greatest ammonium removal rate and maximum biomass were achieved by using sodium pyruvate (7 g/L) as carbon source, C/N 12, pH 7, and temperature 30 °C. Under optimal conditions, the removal rate of ammonia nitrogen reached 96%. Besides, the growth characteristic and the ability of utilizing nitrate and nitrite were investigated. The results demonstrated that strain TN-10 exhibited excellent characteristics to remove both nitrate and nitrite, with the removal rate of 95.44% and 99.87%, respectively. In addition, the nitrite reductase (NiR) and nitrate reductase (NR) involved in denitrification were both active, with the activities of 0.0815 and 0.0283 U/mg proteins, respectively. Furthermore, the aggregation ability, auto-aggregation kinetics, and the relationship between zeta potentials and flocculating efficiency were determined. These results indicated that the strain Klebsiella sp. TN-10, with efficient heterotrophic nitrification-aerobic denitrification ability, has potential application in wastewater treatment.


Klebsiella Nitrogen removal Nitrification Denitrification Aggregation 


Funding Information

This study was funded by the Special Foundation for State Key Research and Development Program of China (2017YFB0308500), the National Natural Science Foundation of China (31671849), and Talent Training platform construction of Sichuan University (SCUKG017).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Supplementary material

12010_2018_2932_MOESM1_ESM.docx (25 kb)
ESM 1 (DOCX 25 kb)


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.College of Light Industry, Textile and Food EngineeringSichuan UniversityChengduChina
  2. 2.Key Laboratory of Leather Chemistry and Engineering, Ministry of EducationSichuan UniversityChengduChina

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