Synthesis of Geraniol Esters in a Continuous-Flow Packed-Bed Reactor of Immobilized Lipase: Optimization of Process Parameters and Kinetic Modeling
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With increasing demand for perfumes, flavors, beverages, and pharmaceuticals, the various associated industries are resorting to different approaches to enhance yields of desired compounds. The use of fixed-bed biocatalytic reactors in some of the processes for making fine chemicals will be of great value because the reaction times could be reduced substantially as well as high conversion and yields obtained. In the current study, a continuous-flow packed-bed reactor of immobilized Candida antarctica lipase B (Novozym 435) was employed for synthesis of various geraniol esters. Optimization of process parameters such as biocatalyst screening, effect of solvent, mole ratio, temperature and acyl donors was studied in a continuous-flow packed-bed reactor. Maximum conversion of ~ 87% of geranyl propionate was achieved in 15 min residence time at 70 °C using geraniol and propionic acid with a 1:1 mol ratio. Novozym 435 was found to be the most active and stable biocatalyst among all tested. Ternary complex mechanism with propionic acid inhibition was found to fit the data.
KeywordsFlow chemistry Biocatalysis Immobilized Candida antarctica lipase B Geraniol Enzyme kinetics
HMS and MPK received JRF and SRF fellowship, respectively, from the University Grants Commission, Government of India under UGC-BSR program. GDY acknowledges support from R.T. Mody Distinguished Professor Endowment and J. C. Bose National Fellowship of Department of Science and Technology (Govt. of India).
Compliance with Ethical Standards
We have complied with all ethical standards.
Conflict of Interest
The authors declare that they have no conflict of interest.
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