Chemical Cell Lysis System Applicable to Lab-on-a-Disc
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The design and fabrication of a heating system has been a significant challenge in implementing chemical lysis on a lab-on-a-disc (LOD). The proposed system contains a sample inlet, phase change material (PCM) array, heating chamber, and valve in a single disc, providing cost-effective, rapid, and fully automated chemical cell lysis. Compared to the conventional cell lysis system, our cell lysis system has many advantages, such as a compact structure that is easily integrated into the LOD and reduced processing time and labor. The experiments are conducted with Salmonella typhimurium strains to demonstrate the performance. The experimental results show that the proposed approach is greatly effective in realizing a chemical cell lysis system on an LOD with higher throughput in terms of purity and yield of DNA.
KeywordsLab on a disk Chemical lysis POCT Cell lysis Microfluidics
All authors acknowledge Mathpower Co., Ltd., Korea, for financial support.
- 5.Moghimi, H., Moradi, A., Hamedi, J., & Basiri, M. (2016). Development of a loop-mediated isothermal amplification assay for rapid and specific identification of ACT producing Alternaria alternata, the agent of brown spot disease in tangerine. Applied Biochemistry and Biotechnology, 178, 1207–1219.CrossRefGoogle Scholar
- 9.Kim, J. T., Jang, S. H., Jia, G. Y., Zoval, J. V., Da Silva, N. A., & Madou, M. J. (2004). Cell lysis on a microfluidic CD (compact disc). LabChip, 4, 516–522.Google Scholar
- 11.Padilla, E., González, V., Manterola, J. M., Lonca, J., Pérez, A., Matas, L., Quesada, M. D., & Ausina, V. (2003). Evaluation of two different cell lysis methods for releasing mycobacterial nucleic acids in the INNO-LiPA mycobacteria test, diagnostic microbiology and infectious disease. Diagnostic Microbiology and Infectious Disease, 46, 19–23.CrossRefGoogle Scholar
- 18.Zhou, Q. J., Wang, L., Chen, J., Wang, R. N., Shi, Y. H., Li, C. H., Zhang, D. M., Yan, X. J., & Zhang, Y. J. (2014). Development and evaluation of a real-time fluorogenic loop-mediated isothermal amplification assay integrated on a microfluidic disc chip (on-chip LAMP) for rapid and simultaneous detection of ten pathogenic bacteria in aquatic animals. Journal of Microbiological Methods, 104, 26–35.CrossRefGoogle Scholar