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Applied Biochemistry and Biotechnology

, Volume 183, Issue 1, pp 20–29 | Cite as

Chemical Cell Lysis System Applicable to Lab-on-a-Disc

  • Dayeseul Lim
  • Jae Chern Yoo
Article

Abstract

The design and fabrication of a heating system has been a significant challenge in implementing chemical lysis on a lab-on-a-disc (LOD). The proposed system contains a sample inlet, phase change material (PCM) array, heating chamber, and valve in a single disc, providing cost-effective, rapid, and fully automated chemical cell lysis. Compared to the conventional cell lysis system, our cell lysis system has many advantages, such as a compact structure that is easily integrated into the LOD and reduced processing time and labor. The experiments are conducted with Salmonella typhimurium strains to demonstrate the performance. The experimental results show that the proposed approach is greatly effective in realizing a chemical cell lysis system on an LOD with higher throughput in terms of purity and yield of DNA.

Keywords

Lab on a disk Chemical lysis POCT Cell lysis Microfluidics 

Notes

Acknowledgements

All authors acknowledge Mathpower Co., Ltd., Korea, for financial support.

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.College of Information & Communication EngineeringSungKyunKwan UniversitySuwonSouth Korea

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