Differential Maturation of miR-17 ~ 92 Cluster Members in Human Cancer Cell Lines
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While some microRNAs are transcribed from a specific promoter, at least one third of human miRNA genes are clustered, wherein multiple miRNA genes are generated from a single primary transcript such as miR-17 ~ 92 cluster. Although six members of the cluster are generated from a single transcript, the mature level of each member may be diverse in various cell types. Here, we attempt to monitor the mature level of miR-17, miR-92a, and miR-20a from miR-17 ~ 92 cluster in blood (HL60 (human promyelocytic leukemia cells) and Jurkat) and breast (MDA-MB-231 and MCF-7) cancer cell lines. Interestingly, different mature levels of the miRNAs were observed in each cell line. While miR-20 was highly matured in HL60 and MDA-MB-231 cell lines, higher mature level of miR-92a was observed in Jurkat cell line compared to that of miR-20 and miR-17. Further, the mature level of miRNAs was also measured in normal and cancer cell lines. Although the mature level of miR-17 and miR-92a increased in HL60 and Jurkat cell lines, miR-20 expression showed an almost identical level in blood cancer cell lines compared to controls. Conversely, miR-20 mature level significantly increased in breast cancer cell lines whereas the expression level of miR-92a was comparable in MDA-MB-231, MCF-7, and MCF-10A cell lines.
KeywordsCancer cell lines miR-17 ~ 92 cluster Stem cell microRNA maturation
This work was supported by the Iran National Science Foundation (INSF), research grants of Shahid Beheshti University of Medical Sciences, Tehran. Drug Applied Research Center, and Tabriz University of Medical Sciences, Tabriz, Iran (Grant No 93/22). We would like to thank the Department of Molecular Biology and Genetic Engineering, Stem Cell Technology Research Center for their kind cooperation in providing materials and equipment. We also thank Ms. Ameneh Kouchaki for technical assistance.
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
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