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Applied Biochemistry and Biotechnology

, Volume 182, Issue 1, pp 181–196 | Cite as

Partial Characterization of the Proteolytic Properties of an Enzymatic Extract From “Aguama” Bromelia pinguin L. Fruit Grown in Mexico

  • Jesús Martín Moreno-Hernández
  • Xitlalli Desideria Hernández-Mancillas
  • Evelia Lorena Coss Navarrete
  • Miguel Ángel Mazorra-Manzano
  • Idalia Osuna-Ruiz
  • Víctor Alfonso Rodríguez-Tirado
  • Jesús Aarón Salazar-Leyva
Article

Abstract

Plant proteases are capable of performing several functions in biological systems, and their use is attractive for biotechnological process due to their interesting catalytic properties. Bromelia pinguin (aguama) is a wild abundant natural resource in several regions of Central America and the Caribbean Islands but is underutilized. Their fruits are rich in proteases with properties that are still unknown, but they represent an attractive source of enzymes for biotechnological applications. Thus, the proteolytic activity in enzymatic crude extracts (CEs) from wild B. pinguin fruits was partially characterized. Enzymes in CEs showed high proteolytic activity at acid (pH 2.0–4.0) and neutral alkaline (pH 7.0–9.0) conditions, indicating that different types of active proteases are present. Proteolytic activity inhibition by the use of specific protease inhibitors indicated that aspartic, cysteine, and serine proteases are the main types of proteases present in CEs. Activity at pH 3.0 was stable in a broad range of temperatures (25–50 °C) and retained its activity in the presence of surfactants (SDS, Tween-80), reducing agents (DTT, 2-mercapoethanol), and organic solvents (methanol, ethanol, acetone, 2-propanol), which suggests that B. pinguin proteases are potential candidates for their application in brewing, detergent, and pharmaceutical industries.

Keywords

Bromelia pinguin Aguama Characterization Enzyme stability Proteolytic activity 

Notes

Acknowledgements

The first author wants to thank the National Council of Science and Technology of Mexico (CONACyT) for the economic support during postgraduate studies. Hernández-Mancillas and Coss-Navarrete wish to thank the “Programa Interinstitucional para el Fortalecimiento de la Investigación y el Posgrado del Pacífico (DELFIN)” for the received summer scholarship. We thank Gerardo Reyna for the bibliography support during this research.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Jesús Martín Moreno-Hernández
    • 1
  • Xitlalli Desideria Hernández-Mancillas
    • 2
  • Evelia Lorena Coss Navarrete
    • 2
  • Miguel Ángel Mazorra-Manzano
    • 3
  • Idalia Osuna-Ruiz
    • 2
  • Víctor Alfonso Rodríguez-Tirado
    • 2
  • Jesús Aarón Salazar-Leyva
    • 2
  1. 1.Programa de Investigación en Biotecnología. Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), Campo Experimental Valle de CuliacánCuliacánMexico
  2. 2.Maestría en Ciencias Aplicadas, Unidad Académica de Ingeniería en BiotecnologíaUniversidad Politécnica de Sinaloa (UPSIN)MazatlánMexico
  3. 3.Laboratorio de Biotecnología de Lácteos, Química y Autenticidad de AlimentosCoordinación de Tecnología de Alimentos de Origen Animal Centro de Investigación en Alimentación y Desarrollo, A.C. (CIAD, A.C.)HermosilloMexico

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