Applied Biochemistry and Biotechnology

, Volume 181, Issue 4, pp 1485–1495 | Cite as

Phytase Production and Development of an Ideal Dephytinization Process for Amelioration of Food Nutrition Using Microbial Phytases

  • Jinender Jain
  • Bijender SinghEmail author


Development of an ideal process for reduction of food phytates using microbial phytases is a demanding task by all food and feed industries all over the world. Phytase production by Bacillus subtilis subsp. subtilis JJBS250 isolated from soil sample was optimized in submerged fermentation using statistical tools. Among all the culture variables tested, sucrose, sodium phytate and Tween-80 were identified as the most significant variables using the Placket–Burman design. Further optimization of these variables resulted in a 6.79-fold improvement in phytase production (7170 U/L) as compared to unoptimized medium. Supplementation of microbial phytases (fungal and bacterial) resulted in improved bioavailability of nutritional components with the concomitant liberation of inorganic phosphorus, reducing sugar, soluble protein and amino acids, thus mitigating anti-nutritional properties of phytic acid.


Phytase Bacillus subtilis subsp. subtilis JJBS250 Sporotrichum thermophile Response surface methodology Dephytinization 



Authors are highly thankful to the Department of Science and Technology (No. SR/FT/LS-95/2010), New Delhi, India for providing the financial assistance during this research work.


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Laboratory of Bioprocess Technology, Department of MicrobiologyMaharshi Dayanand UniversityRohtakIndia

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