Applied Biochemistry and Biotechnology

, Volume 181, Issue 2, pp 844–859 | Cite as

Use of Swine Wastewater as Alternative Substrate for Mycelial Bioconversion of White Rot Fungi

  • Jangwoo Lee
  • Seung Gu Shin
  • Jinmo Ahn
  • Gyuseong Han
  • Kwanghyun Hwang
  • Woong Kim
  • Seokhwan HwangEmail author


Seven white rot fungal species were tested for growth as mycelia using swine wastewater (SW), an agro-waste with tremendous environmental footprint, as the sole nutrient source. The SW contained high concentrations of carbon and nitrogen components, which could support nutritional requirements for mycelial growth. Out of the seven species, Pleurotus ostreatus and Hericium erinaceus were successfully cultivated on the SW medium using solid-state fermentation. Response surface methodology was employed to determine the combination of pH, temperature (T), and substrate concentration (C) that maximizes mycelial growth rate (Kr) for the two species. The optimum condition was estimated as pH = 5.8, T = 28.8 °C, and C = 11.2 g chemical oxygen demand (COD)/L for P. ostreatus to yield Kr of 11.0 mm/day, whereas the greatest Kr (3.1 mm/day) was anticipated at pH = 4.6, T = 25.5 °C, and C = 11.9 g COD/L for H. erinaceus. These Kr values were comparable to growth rates obtained using other substrates in the literature. These results demonstrate that SW can be used as an effective substrate for mycelial cultivation of the two white rot fungal species, suggesting an alternative method to manage SW with the production of potentially valuable biomass.


White rot fungi Mycelium Bioconversion Solid-state fermentation Optimization Response surface methodology 



This work was financially supported by Korea Ministry of Environment as “Knowledge based environmental service (Waste to energy recycling) Human resource development Project.” This work was also supported by “Human Resources Program in Energy Technology” of the Korea Institute of Energy Technology Evaluation and Planning (KETEP) Grant, funded by the Ministry of Trade, Industry and Energy, Republic of Korea (No. 20144030200460).

Supplementary material

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Suppl. Fig. 1 (DOCX 236 kb)
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Suppl. Fig. 2 (DOCX 18 kb)
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Suppl. Fig. 3 (DOCX 18 kb)


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Jangwoo Lee
    • 1
  • Seung Gu Shin
    • 1
  • Jinmo Ahn
    • 1
    • 2
  • Gyuseong Han
    • 1
  • Kwanghyun Hwang
    • 3
  • Woong Kim
    • 4
  • Seokhwan Hwang
    • 1
    Email author
  1. 1.School of Environmental Science and EngineeringPohang University of Science and Technology (POSTECH)PohangRepublic of Korea
  2. 2.Division of Advanced Nuclear EngineeringPOSTECHPohangRepublic of Korea
  3. 3.Environmental Process Engineering Team, Global Engineering Division, GS E&CSeoulRepublic of Korea
  4. 4.Department of Environmental EngineeringKyungpook National UniversityDaeguSouth Korea

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