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Applied Biochemistry and Biotechnology

, Volume 181, Issue 1, pp 32–47 | Cite as

Variation in the Gut Microbiota of Termites (Tsaitermes ampliceps) Against Different Diets

  • Lijuan Su
  • Lele Yang
  • Shi Huang
  • Yan Li
  • Xiaoquan Su
  • Fengqin Wang
  • Cunpei Bo
  • En Tao WangEmail author
  • Andong SongEmail author
Article

Abstract

Termites are well recognized for their thriving on recalcitrant lignocellulosic diets through nutritional symbioses with gut-dwelling microbiota; however, the effects of diet changes on termite gut microbiota are poorly understood, especially for the lower termites. In this study, we employed high-throughput 454 pyrosequencing of 16S V1–V3 amplicons to compare gut microbiotas of Tsaitermes ampliceps fed with lignin-rich and lignin-poor cellulose diets after a 2-week-feeding period. As a result, the majority of bacterial taxa were shared across the treatments with different diets, but their relative abundances were modified. In particular, the relative abundance was reduced for Spirochaetes and it was increased for Proteobacteria and Bacteroides by feeding the lignin-poor diet. The evenness of gut microbiota exhibited a significant difference in response to the diet type (filter paper diets < corn stover diets < wood diets), while their richness was constant, which may be related to the lower recalcitrance of this biomass to degradation. These results have important implications for sampling and analysis strategies to probe the lignocellulose degradation features of termite gut microbiota and suggest that the dietary lignocellulose composition could cause shifting rapidly in the termite gut microbiota.

Keywords

Tsaitermes ampliceps Gut symbionts Lignocellulose degradation Corn stover Filter paper 454 Pyrosequencing 

Notes

Acknowledgments

This research was supported by National Science Foundation of China Grant 31170350.

Compliance with Ethical Standards

Conflict of Interest

We declare that we have no financial and personal relationships with other people or organizations that can inappropriately influence our work. There is no professional or other personal interest of any nature or kind in any product, service, and/or company that could be construed as influencing the position presented in the manuscript entitled, “Variation in the gut microbiota and sensitivity to dietary changes in termite hosts”.

Supplementary material

12010_2016_2197_MOESM1_ESM.docx (156 kb)
Supplementary Fig. S1 (DOCX 156 kb)
12010_2016_2197_MOESM2_ESM.docx (29 kb)
Supplementary Table S1 (DOCX 29 kb)
12010_2016_2197_MOESM3_ESM.docx (69 kb)
Supplementary Table S2 (DOCX 68 kb)

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Lijuan Su
    • 1
  • Lele Yang
    • 1
  • Shi Huang
    • 2
  • Yan Li
    • 1
  • Xiaoquan Su
    • 2
  • Fengqin Wang
    • 1
    • 3
  • Cunpei Bo
    • 2
  • En Tao Wang
    • 4
    Email author
  • Andong Song
    • 1
    • 3
    • 5
    Email author
  1. 1.College of Life SciencesHenan Agricultural UniversityZhengzhouChina
  2. 2.BioEnergy Genome Center, CAS Key Laboratory of Biofuels and Shandong Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of SciencesQingdaoChina
  3. 3.Key Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of AgricultureZhengzhouChina
  4. 4.Departamento de Microbiología, Escuela Nacional de Ciencias BiológicasInstituto Politécnico NacionalMéxico D.F.Mexico
  5. 5.ZhengzhouChina

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