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Applied Biochemistry and Biotechnology

, Volume 180, Issue 8, pp 1508–1523 | Cite as

Understanding the Nonproductive Enzyme Adsorption and Physicochemical Properties of Residual Lignins in Moso Bamboo Pretreated with Sulfuric Acid and Kraft Pulping

  • Caoxing Huang
  • Juan He
  • Douyong Min
  • Chenhuan Lai
  • Qiang Yong
Article

Abstract

In this work, to elucidate why the acid-pretreated bamboo shows disappointingly low enzymatic digestibility comparing to the alkali-pretreated bamboo, residual lignins in acid-pretreated and kraft pulped bamboo were isolated and analyzed by adsorption isotherm to evaluate their extents of nonproductive enzyme adsorption. Meanwhile, physicochemical properties of the isolated lignins were analyzed and a relationship was established with non-productive adsorption. Results showed that the adsorption affinity and binding strength of cellulase on acid-pretreated bamboo lignin (MWLa) was significantly higher than that on residual lignin in pulped bamboo (MWLp). The maximum adsorption capacity of cellulase on MWLp was 129.49 mg/g lignin, which was lower than that on MWLa (160.25 mg/g lignin). When isolated lignins were added into the Avicel hydrolysis solution, the inhibitory effect on enzymatic hydrolysis efficiency of MWLa was found to be considerably stronger than that with MWLp. The cellulase adsorption on isolated lignins was correlated positively with hydrophobicity, phenolic hydroxyl group, and degree of condensation but negatively with surface charges and aliphatic hydroxyl group. These results suggest that the higher nonproductive cellulase adsorption and physicochemical properties of residual lignin in acid-pretreated bamboo may be responsible for its disappointingly low enzymatic digestibility.

Keywords

Moso bamboo Pretreatment Nonproductive adsorption Adsorption isotherm Physicochemical properties 

Notes

Acknowledgments

The research was supported by the National Natural Science Foundation of China (31570561) and the Natural Science Foundation of Jiangsu Province (BK20150874). The authors thank the Priority Academic Program Development of Jiangsu Higher Education Institution (PAPD) and the Doctorate Fellowship Foundation of Nanjing Forestry University for supporting the work presented in this paper.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Caoxing Huang
    • 1
    • 2
  • Juan He
    • 1
    • 2
  • Douyong Min
    • 3
  • Chenhuan Lai
    • 1
    • 2
  • Qiang Yong
    • 1
    • 2
  1. 1.Co-Innovation Center for Efficient Processing and Utilization of Forest ProductsNanjing Forestry UniversityNanjingChina
  2. 2.College of Chemical EngineeringNanjing Forestry UniversityNanjingChina
  3. 3.College of Light Industry Science and EngineeringNanjing Forestry UniversityNanjingChina

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