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Applied Biochemistry and Biotechnology

, Volume 180, Issue 7, pp 1328–1344 | Cite as

Heavy Metal Resistances and Chromium Removal of a Novel Cr(VI)-Reducing Pseudomonad Strain Isolated from Circulating Cooling Water of Iron and Steel Plant

  • Jian-Kun ZhangEmail author
  • Zhen-Hua Wang
  • Yun Ye
Article

Abstract

Three bacterial isolates, GT2, GT3, and GT7, were isolated from the sludge and water of a circulating cooling system of iron and steel plant by screening on Cr(VI)-containing plates. Three isolates were characterized as the members of the genus Pseudomonas on the basis of phenotypic characteristics and 16S rRNA sequence analysis. All isolates were capable of resisting multiple antibiotics and heavy metals. GT7 was most resistant to Cr(VI), with a minimum inhibitory concentration (MIC) of 6.5 mmol L−1. GT7 displayed varied rates of Cr(VI) reduction in M2 broth, which was dependent on pH, initial Cr(VI) concentration, and inoculating dose. Total chromium analysis revealed that GT7 could remove a part of chromium from the media, and the maximum rate of chromium removal was up to 40.8 %. The Cr(VI) reductase activity of GT7 was mainly associated with the soluble fraction of cell-free extracts and reached optimum at pH 6.0∼8.0. The reductase activity was apparently enhanced by external electron donors and Cu(II), whereas it was seriously inhibited by Hg(II), Cd(II), and Zn(II). The reductase showed a K m of 74 μmol L−1 of Cr(VI) and a V max of 0.86 μmol of Cr(VI) min−1 mg−1 of protein. The results suggested that GT7 could be a promising candidate for in situ bioremediation of Cr(VI).

Keywords

Pseudomonas sp. Heavy metal resistance Chromate reduction Chromium removal Chromate reductase Bioremediation 

Notes

Acknowledgments

This study was supported by the Fundamental Research Funds for the Central Universities, Wuhan University of Technology, Wuhan, Hubei, P. R. China (2011-IA-032) and by the Research and Development Project, General Administration of Quality Supervision, Inspection and Quarantine of the PRC, Beijing, P. R. China (2015IK123).

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Department of Biological Science and Biotechnology, School of Chemistry, Chemical Engineering and Life SciencesWuhan University of TechnologyWuhanPeople’s Republic of China
  2. 2.Technological Center of Inspection and Quarantine, Hubei Entry-Exit Inspection and Quarantine BureauWuhanPeople’s Republic of China

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