Structural Characterization of a Novel Antioxidant Pigment Produced by a Photochromogenic Microbacterium oxydans Strain
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The Microbacteriaceae family, such as Microbacterium, is well known for its ability to produce carotenoid-type pigments, but little has been published on the structure of such pigments. Here, we isolated the yellow pigment that is responsible for the yellowish color of a Microbacterium oxydans strain isolated from a decomposing stump of a resinous tree. The pigment, which is synthesized when the bacterium is grown under light, was purified and characterized using several spectroscopic analyses, such as ultraviolet-visible spectroscopy (UV–Vis), Fourier transform infrared spectroscopy (FTIR), 1H and 13C nuclear magnetic resonance (1H NMR, 13C NMR), and high-resolution mass spectrometry (HRMS). From these analysis, a molecular formula (C27H42O2) and a chemical structure (8-hydroxymethyl-2,4,12-trimethyl-14-(2,6,6-trimethyl-cyclohex-2-enyl)-teradeca-3,7,9,11,13-pentan-2-ol) were deduced. The chemical properties of the pigment, such as aqueous stability at different pH, stability in different organic solvents, and antioxidant capacity, are also reported. Together, these data and previous studies have resulted in the identification of a new antioxidant pigment produced by M. oxydans. To the best of our knowledge, this is the first thorough investigation of this carotenoid-like pigment in the Microbacterium genera.
KeywordsCarotenoid Spectroscopy Microbacterium oxydans Photochromogenic bacteria Culture Wood decay
MB gratefully acknowledges the financial support from the Natural Sciences and Engineering Research Council of Canada (NSERC) grant no.138654. The editorial help of Dr. Martin Kalmokoff in preparing this manuscript is acknowledged.
Compliance with Ethical Standards
This article does not contain any studies with human participants or animals performed by any of the authors.
Conflict of Interest
The authors declare that they have no conflicts of interests.
- 2.Babitha, S. (2009). Biotechnology for agro-industrial residues utilisation. In P. S. Nigam & A. Pandey (Eds.), Chap 8: Microbial pigments. Berlin: Springer.Google Scholar
- 11.Sasidharan, P., Raja, R., Karthik, C., Ranandkumar, S., & Indra, A. P. (2013). Isolation and characterization of yellow pigment producing Exiguobacterium sps. Journal of Biomolecular Techniques, 4(4), 632–635.Google Scholar
- 16.Dufossé, L. (2006). Microbial production of food grade pigments. Food Technology and Biotechnology, 44(3), 313–321.Google Scholar
- 17.Meddeb-Mouelhi, F., Moisan, J. K., & Beauregard, M. (2015). Characterization of bacteria community isolated from wood decay. The Biochemical Journal, 10(1), 1–14.Google Scholar
- 21.Godinho, A., & Bhosle, S. (2008). Carotenes produced by alkaliphilic orange-pigmented strain of Microbacterium arborescens—AGSB isolated from coastal sand dunes. Indian Journal of Marine Science, 37(3), 307–312.Google Scholar
- 22.Engler, G. (1985). NMR of carotenoids new experimental techniques. Pure and Applied Chemistry, 57(5), 801–821.Google Scholar
- 24.Whitman, W., Goodfellow, M., Kämpfer, P., Busse, H. J., Trujillo, M., Ludwig, W., & Suzuki, K. (2012). Bergey’s manual of systematic bacteriology volume 5: the Actinobacteria. New-York: Springer.Google Scholar
- 27.Wieser, M., Schumann, P., Martin, K., Altenburger, P., Burghardt, J., Lubitz, W., & Busse, H. J. (1999). Agrococcus citreus sp. nov. isolated from a medieval wall painting of the chapel of Castle Herberstein (Austria). International Journal of Systematic Bacteriology, 49, 1165–1170.CrossRefGoogle Scholar