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Applied Biochemistry and Biotechnology

, Volume 180, Issue 4, pp 766–779 | Cite as

Regeneration-Based Quantification of Coumarins (Scopoletin and Scoparone) in Abutilon indicum In Vitro Cultures

  • Kiranmayee RaoEmail author
  • Bhuvaneswari Chodisetti
  • Suryakala Gandi
  • Archana Giri
  • P. B. Kavi Kishor
Article

Abstract

Abutilon indicum exploited for its immense value has been propagated successfully through multiple shoot induction and somatic embryogenesis. Direct regeneration (8.20 ± 0.83 shoots) was achieved from nodal explants using 0.5 mg/l kinetin (Kn) in MS media. The basal callus from nodal explants turned embryogenic on subsequent introduction of 0.2 mg/l TDZ into the Kn-supplemented media, giving rise to somatic embryos. The embryogenic potential of calli expressed in terms of embryo-forming capacity (EFC) increased from 8.15 EFC to 20.95 EFC after plasmolysis. The phytochemical analysis (HPLC) for the presence of scopoletin and scoparone has revealed a unique accumulation pattern, with higher levels of scopoletin during the earlier stages and scoparone in the later stages of development. The embryogenic calli contained the highest amount of coumarins (99.20 ± 0.97 and 61.03 ± 0.47 μg/gFW, respectively) followed by regenerated plant (9.43 ± 0.20 and 36.36 ± 1.19 μg/gFW, respectively), obtained via somatic embryogenesis. Rapid multiplication of A. indicum equipped with two potent coumarins is important in order to meet the commercial demand for combat against dreadful diseases, thereby providing a new platform for plant-based drugs and their manufacture on a commercial scale.

Keywords

A. indicum Coumarins Scopoletin Scoparone Somatic embryogenesis HPLC 

Notes

Acknowledgments

This work was funded by the University Grants Commission (UGC) in the form of Dr. D.S. Kothari postdoctoral fellowship to Dr. Kiranmayee Rao under the grant number [no.F.4-2/2006(BSR)/13-738/2012(BSR)].

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Kiranmayee Rao
    • 1
    Email author
  • Bhuvaneswari Chodisetti
    • 1
  • Suryakala Gandi
    • 2
  • Archana Giri
    • 2
  • P. B. Kavi Kishor
    • 1
  1. 1.Department of Genetics, Faculty of ScienceOsmania UniversityHyderabadIndia
  2. 2.Centre for BiotechnologyJawaharlal Nehru Technological University HyderabadHyderabadIndia

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