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Applied Biochemistry and Biotechnology

, Volume 180, Issue 4, pp 638–654 | Cite as

Bio-therapeutic Potential and Cytotoxicity Assessment of Pectin-Mediated Synthesized Nanostructured Cerium Oxide

  • Sandeep N. Patil
  • Jayasinh S. Paradeshi
  • Prapti B. Chaudhari
  • Satyendra J. Mishra
  • Bhushan L. ChaudhariEmail author
Article

Abstract

In the present studies, renewable and nontoxic biopolymer, pectin, was extracted from Indian red pomelo fruit peels and used for the synthesis of cerium oxide nanoparticles (CeO2-NPs) having bio-therapeutic potential. The structural information of extracted pectin was investigated by FTIR and NMR spectroscopic techniques. Physicochemical characteristics of this pectin suggested its application in the synthesis of metal oxide nanoparticles. Using this pectin as a template, CeO2-NPs were synthesized by simple, one step and eco-friendly approach. The UV–Vis spectrum of synthesized CeO2-NPs exhibited a characteristic absorption peak at wavelength 345 nm, which can be assigned to its intrinsic band gap (3.59 eV) absorption. Photoluminescence measurements of CeO2-NPs revealed that the broad emission was composed of seven different bands. FTIR analysis ensured involvement of pectin in the formation and stabilization of CeO2-NPs. FT-Raman spectra showed a sharp Raman active mode peak at 461.8 cm−1 due to a symmetrical stretching mode of Ce–O vibration. DLS, FESEM, EDX, and XRD analysis showed that the CeO2-NPs prepared were polydispersed, spherical shaped with a cubic fluorite structure and average particle size ≤40 nm. These CeO2-NPs displayed broad spectrum antimicrobial activity, antioxidant potential, and non-cytotoxic nature.

Keywords

Pectin Indian red pomelo fruit peels Cerium oxide nanoparticles Antioxidant Antibacterial Non-cytotoxic 

Notes

Acknowledgment

SNP and JSP are thankful to the UGC, New Delhi for UGS-BSR/RFSMS fellowships. PBC is grateful to the DST, New Delhi for INSPIRE fellowship. All the authors acknowledge the SAIF-Chandigarh for NMR spectroscopic analysis, SAIF-IIT Chennai for FT-Raman spectroscopic study and UGS-SAP-DRS III and DST-FIST for providing infrastructure at School of Life Sciences of North Maharashtra University, Jalgaon.

Supplementary material

12010_2016_2121_MOESM1_ESM.docx (866 kb)
ESM 1 (DOCX 866 kb)

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Sandeep N. Patil
    • 1
  • Jayasinh S. Paradeshi
    • 1
  • Prapti B. Chaudhari
    • 2
  • Satyendra J. Mishra
    • 2
  • Bhushan L. Chaudhari
    • 1
    Email author
  1. 1.Department of Microbiology, School of Life SciencesNorth Maharashtra UniversityJalgaonIndia
  2. 2.Department of Polymer Science and TechnologyUniversity Institute of Chemical Technology, North Maharashtra UniversityJalgaonIndia

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