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Applied Biochemistry and Biotechnology

, Volume 177, Issue 7, pp 1466–1479 | Cite as

2-Acetyl-1-Pyrroline Augmentation in Scented indica Rice (Oryza sativa L.) Varieties Through Δ1-Pyrroline-5-Carboxylate Synthetase (P5CS) Gene Transformation

  • Kayghobad Kaikavoosi
  • Trupti D. Kad
  • Rahul L. Zanan
  • Altafhusain B. NadafEmail author
Article

Abstract

2-Acetyl-1-pyrroline (2AP) has been identified as a principal aroma compound in scented rice varieties. Δ1-Pyrroline-5-carboxylate synthetase (P5CS) gene is reported to regulate the proline synthesis in plants and acts as the precursor of 2AP. Two scented indica rice varieties, namely Ambemohar 157 and Indrayani, were subjected to Agrobacterium tumefaciens-mediated genetic transformation containing P5CS gene. Overexpression of P5CS led to a significant increase in proline, P5CS enzyme activity and 2AP levels in transgenic calli, vegetative plant parts, and seeds over control in both the varieties. 2AP level increased more than twofold in transgenic seeds in both varieties. This is the first report of enhancement in 2AP content through overexpression of using P5CS gene, indicating the role of proline as a precursor amino acid in the biosynthesis of 2AP in scented rice.

Keywords

Scented indica rice 2-Acetyl-1-pyrroline Δ1-Pyrroline-5-carboxylate synthetase (P5CSGenetic transformation Proline Volatile compounds 

Abbreviations

P5C

1-Pyrroline-5-carboxylic acid

2, 4-D

2,4-Dichlorophenoxyacetic acid

2AP

2-Acetyl-1-pyrroline

BAP

Benzylaminopurine

BADH2

Betaine aldehyde dehydrogenase 2

GC-FID

Gas chromatography–flame-ionized detector

GC–MS

Gas chromatography–mass spectrometry

GSA

Glutamic γ-semialdehyde

HS-SPME

Headspace–solid phase micro-extraction

LB

Luria broth

NAA

Naphthalene acetic acid

RNAi

RNA interference

GABald

γ-Aminobutyraldehyde

GABA

γ-Aminobutyric acid

P5CS

Δ1-Pyrroline-5-carboxylate synthetase

Notes

Acknowledgments

The authors thank Dr. A. Karkhane, National Institute for Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran, for providing the pBI121-P5CS vector. The authors are thankful to Dr. P. Srinivas (Central Food and Technology Research Institute, Mysore, India) for the generous gift of authentic 2AP.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Kayghobad Kaikavoosi
    • 1
  • Trupti D. Kad
    • 1
  • Rahul L. Zanan
    • 1
  • Altafhusain B. Nadaf
    • 1
    Email author
  1. 1.Department of BotanySavitribai Phule Pune UniversityPuneIndia

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