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Applied Biochemistry and Biotechnology

, Volume 175, Issue 8, pp 3800–3812 | Cite as

Manganese Peroxidases from Ganoderma applanatum Degrade β-Carotene Under Alkaline Conditions

  • Isabel LanfermannEmail author
  • Diana Linke
  • Manfred Nimtz
  • Ralf G. Berger
Article

Abstract

A β-carotene-degrading enzyme activity was observed in liquid cultures of the basidiomycete Ganoderma applanatum. Supplementing the cultures with β-carotene induced the bleaching activity. Purification via hydrophobic interaction, ion exchange and size exclusion chromatography followed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) resulted in a single protein band. LC-ion-trap-MS analyses and gene amplification identified two manganese peroxidase isoenzymes with 97.8 % identity on the amino acid level. These showed an estimated molecular mass of 48 kDa and an isoelectric point of 2.6. Properties not yet described for other manganese peroxidases were hydrogen-peroxide-independent catalysis and two maxima of the bleaching activity, a distinct one at pH 5 and a lower one at pH 8. During simulated washing studies, the applicability of the isoenzymes for the brightening of carotenoids under alkaline conditions was proven. The new enzymes may replace common bleaching agents to produce environmentally more compatible detergent formulations.

Keywords

Biocatalysis Enzyme activity Carotene degradation Manganese peroxidase Purification Filamentous fungi Ganoderma applanatum Washing Alkaline activity 

Abbreviations

CD-activity

Carotene-degrading activity

HIC

Hydrophobic interaction chromatography

SEC

Size exclusion chromatography

MS

Mass spectrometry

Gap

Ganoderma applanatum

MnP

Manganese peroxidase

Notes

Acknowledgments

The authors gratefully acknowledge the essential support from Henkel AG & Co. KGaA, 328 Düsseldorf, Germany. Furthermore, we wish to thank R. H. Leonhardt for his valuable assistance in molecular biology.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Isabel Lanfermann
    • 1
    Email author
  • Diana Linke
    • 1
  • Manfred Nimtz
    • 2
  • Ralf G. Berger
    • 1
  1. 1.Institut für LebensmittelchemieLeibniz Universität HannoverHannoverGermany
  2. 2.Helmholtz Zentrum für InfektionsforschungBraunschweigGermany

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