Improving Cell Growth and Lipid Accumulation in Green Microalgae Chlorella sp. via UV Irradiation
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Microalgae with high biomass and high lipid content are the ideal feedstock for biodiesel production. To obtain such microalgae, ultraviolet (UV) irradiation was applied to Chlorella sp. to induce mutagenesis. The growth characteristics, total nitrogen (TN), and biochemical compositions of the control and UV mutation strains were analyzed. Compared to the control strain, the biomass for the UV mutation strain was 7.6 % higher and it presented a higher growth rate. The lipid content of the UV mutation strain showed different levels of increase and reached the maximum value of 28.1 % on day 15. Furthermore, the lipid productivity of the UV mutation strain showed a desired increase. The nitrogen consumption and Acetyl-CoA carboxylase (ACC) activity contributed to the lipid production by UV. All these results indicate that UV mutagenesis is an efficient method to improve probability for using Chlorella sp. as the potential raw material for biodiesel production.
KeywordsMicroalgae Radiation UV mutagenesis Lipid Biodiesel
This work was supported by the Open Project of State Key Laboratory of Urban Water Resource and Environment (No. HC201323) and National Natural Science Foundation of China (Nos. 50809037, 41430644, and 41273126), Program for Changjiang Scholars and Innovative Research Team in University (No. IRT13078), and Science and Technology Commission of Shanghai Municipality (13230500600), the special S&T project on treatment and control of water pollution (No. 2012ZX07201-003). The authors extend their sincere thanks to all the people in the Key Laboratory of Harbin Institute of Technology.
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