Applied Biochemistry and Biotechnology

, Volume 175, Issue 5, pp 2528–2541 | Cite as

Enhancement of Thermostability and Kinetic Efficiency of Aspergillus niger PhyA Phytase by Site-Directed Mutagenesis

  • Ardeshir Hesampour
  • Seyed Ehsan Ranaei Siadat
  • Mohammad Ali Malboobi
  • Nooshin Mohandesi
  • Seyed Shahriar Arab
  • Mohammad Mehdi Ghahremanpour
Article

Abstract

Phytase efficiently catalyzes the hydrolysis of phytate to phosphate; it can be utilized as an animal supplement to provide animals their nutrient requirements for phosphate and to mitigate environmental pollution caused by unutilized feed phosphate. Owing to animal feed being commonly pelleted at 70 to 90 °C, phytase with a sufficiently high thermal stability is desirable. Based on the crystal structure of PhyA and bioinformatics analysis at variant heat treatments, 12 single and multiple mutants were introduced by site-directed mutagenesis in order to improve phytase thermostability. Mutated constructs were expressed in Pichia pastoris. The manipulated phytases were purified; their biochemical and kinetic investigation revealed that while the thermostability of six mutants was improved, P9 (T314S Q315R V62N) and P12 (S205N S206A T151A T314S Q315R) showed the highest heat stability (P < 0.05) with 24 and 22.6 % greater retention, respectively, compared with the PhyA of the wild type at 80 °C. The Km value of the improved thermostable P9 and P12 mutant enzymes for sodium phytate were 35 and 20 % lower (P < 0.05) with respect to the wild-type enzyme. In conclusion, it is feasible to simultaneously improve the thermostability and the catalytic efficiency of phytase to be used as an animal feed supplement.

Keywords

Aspergillus niger Phytase engineering Thermostabilty Pichia pastoris Molecular dynamic 

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Ardeshir Hesampour
    • 1
  • Seyed Ehsan Ranaei Siadat
    • 2
  • Mohammad Ali Malboobi
    • 3
  • Nooshin Mohandesi
    • 3
  • Seyed Shahriar Arab
    • 4
  • Mohammad Mehdi Ghahremanpour
    • 5
  1. 1.Department of BiologyCentral Tehran Branch, Islamic Azad UniversityTehranIslamic Republic of Iran
  2. 2.Protein Research CentreShahid Beheshti UniversityTehranIslamic Republic of Iran
  3. 3.Department of Plant BiotechnologyNational Institute of Genetic Engineering and Biotechnology (NIGEB)TehranIslamic Republic of Iran
  4. 4.Department of Biophysics, School of Biological SciencesTarbiat Modares UniversityTehranIran
  5. 5.Department of Bioinformatics, School of Biological SciencesInstitute for Research in Fundamental Sciences (IPM)TehranIran

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