Applied Biochemistry and Biotechnology

, Volume 175, Issue 4, pp 2000–2011 | Cite as

Simultaneous Heterotrophic Nitrification and Aerobic Denitrification by the Marine Origin Bacterium Pseudomonas sp. ADN-42

  • Ruofei JinEmail author
  • Tianqi Liu
  • Guangfei Liu
  • Jiti Zhou
  • Jianyu Huang
  • Aijie WangEmail author


Recent research has highlighted the existence of some bacteria that are capable of performing heterotrophic nitrification and have a phenomenal ability to denitrify their nitrification products under aerobic conditions. A high-salinity-tolerant strain ADN-42 was isolated from Hymeniacidon perleve and found to display high heterotrophic ammonium removal capability. This strain was identified as Pseudomonas sp. via 16S rRNA gene sequence analysis. Gene cloning and sequencing analysis indicated that the bacterial genome contains N2O reductase function (nosZ) gene. NH3-N removal rate of ADN-42 was very high. And the highest removal rate was 6.52 mg/L · h in the presence of 40 g/L NaCl. Under the condition of pure oxygen (DO >8 mg/L), NH3-N removal efficiency was 56.9 %. Moreover, 38.4 % of oxygen remained in the upper gas space during 72 h without greenhouse gas N2O production. Keeping continuous and low level of dissolved oxygen (DO <3 mg/L) was helpful for better denitrification performance. All these results indicated that the strain has heterotrophic nitrification and aerobic denitrification abilities, which guarantee future application in wastewater treatment.


Pseudomonas sp. ADN-42 Heterotrophic nitrification Aerobic denitrification N2DO 



This work was supported by the Open Project of State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology (QA201313) and the Fundamental Research Funds for the Central Universities (DUT14LAB09).


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© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and TechnologyDalian University of TechnologyDalianChina
  2. 2.State Key Laboratory of Urban Water Resource and EnvironmentHarbin Institute of TechnologyHarbinChina

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