Applied Biochemistry and Biotechnology

, Volume 174, Issue 3, pp 911–925

Synthesis and Characterization of Reduced Graphene Oxide Supported Gold Nanoparticles-Poly(Pyrrole-Co-Pyrrolepropylic Acid) Nanocomposite-Based Electrochemical Biosensor

  • Nidhi Puri
  • Asad Niazi
  • Avanish K. Srivastava
  • Rajesh
Article

DOI: 10.1007/s12010-014-0997-9

Cite this article as:
Puri, N., Niazi, A., Srivastava, A.K. et al. Appl Biochem Biotechnol (2014) 174: 911. doi:10.1007/s12010-014-0997-9

Abstract

A conducting poly(pyrrole-co-pyrrolepropylic acid) copolymer nanocomposite film (AuNP-PPy-PPa) incorporating gold nanoparticles (AuNP) was electrochemically grown using a single step procedure over electrochemically reduced graphene oxide (RGO) flakes deposited on a silane-modified indium-tin-oxide (ITO) glass plate. The RGO support base provided excellent mechanical and chemical stability to the polymer nanocomposite matrix. The porous nanostructure of AuNP-PPy-PPa/RGO provided a huge accessible area to disperse AuNP, and it avoided metallic agglomeration within the polymer matrix. The AuNP-PPy-PPa/RGO was characterized by high-resolution transmission electron microscopy (HRTEM), contact angle measurements, Fourier transform infrared spectroscopy (FTIR), and electrochemical techniques. The pendant carboxyl group of AuNP-PPy-PPa/RGO was covalently bonded with myoglobin protein antibody, Ab-Mb, for the construction of a bioelectrode. Electrochemical impedance spectroscopy technique was used for the characterization of the bioelectrode and as an impedimetric biosensor for the detection of human cardiac biomarker, Ag-cMb. The bioelectrode exhibited a linear impedimetric response to Ag-cMb in the range of 10 ng mL−1 to 1 μg mL−1, in phosphate-buffered solution (PBS) (pH 7.4, 0.1 M KCl) with a sensitivity of 92.13 Ω cm2 per decade.

Keywords

Conducting polymer Electrochemical sensing Graphene Immunoreaction Antibody 

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Nidhi Puri
    • 1
    • 2
  • Asad Niazi
    • 2
  • Avanish K. Srivastava
    • 1
  • Rajesh
    • 1
  1. 1.CSIR-National Physical LaboratoryNew DelhiIndia
  2. 2.Department of Physics, Faculty of Natural SciencesJamia Millia IslamiaNew DelhiIndia

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