Applied Biochemistry and Biotechnology

, Volume 173, Issue 6, pp 1495–1510 | Cite as

Utilization of Agricultural Residues of Pineapple Peels and Sugarcane Bagasse as Cost-Saving Raw Materials in Scenedesmus acutus for Lipid Accumulation and Biodiesel Production

  • Panida Rattanapoltee
  • Pakawadee Kaewkannetra


The aim of this study is to optimize the lipid accumulation in microalgae by using two agricultural residues of pineapple peels and sugarcane bagasse as low-cost organic carbon sources. Green microalgae Scenedesmus acutus was isolated and selected for cultivation. Effects of three initial sugar concentrations and the stage for adding sugar during cultivation on biomass and lipid production were investigated. The results clearly showed that two-stage cultivation is more suitable than one-stage. The maximum biomass concentration and productivity were obtained at 3.85 g/L and 160.42 mg/L/day when sugarcane bagasse was used. The highest lipid content and lipid yield was reached at 28.05 % and 0.93 g/L when pineapple peels were used, while in the case of sugarcane bagasse, 40.89 % and 1.24 g/L lipid content and yield were obtained. Lipid content was found in normal condition (autotrophic) at 17.71 % which was approximately 2.13-fold lower than when sugarcane bagasse was used (40.89 %). Biodiesel production via in situ transesterification was also investigated; the main fatty acids of palmitic acid and oleic acid were found. This work indicates that using agricultural residues as organic carbon sources could be able to increase lipid content and reduce the cost of biofuel production.


Microalgae Scenedesmus acutus Agricultural residues Pineapple peels Sugarcane bagasse Lipid Biodiesel 


S. acutus

Scenedesmus acutus


Pineapple peels


Sugarcane bagasse


Agricultural residues


Autotrophic cultivation


Mixotrophic cultivation


Specific growth rate


Fatty acid methyl ester



The authors would like to sincerely acknowledge the National Research University (NRU) Project, Khon Kaen University, Khon Kaen 40002, Thailand, for financial contribution under contract project number Ph.D 54302 for the year 2011–2014. In addition, one of the authors (P. Kaewkannetra) also would like to thanks Centre for Alternative Energy Research and Development (AERD), Faculty of Engineering, Khon Kaen University, Khon Kaen, Thailand for some matching fund (contract no. R06/56).


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Graduate SchoolKhon Kaen UniversityKhon KaenThailand
  2. 2.Department of Biotechnology, Faculty of TechnologyKhon Kaen UniversityKhon KaenThailand
  3. 3.Centre for Alternative Energy Research and Development (AERD), Faculty of EngineeringKhon Kaen UniversityKhon KaenThailand

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