Applied Biochemistry and Biotechnology

, Volume 173, Issue 6, pp 1553–1563 | Cite as

Genome Shuffling and Ribosome Engineering of Streptomyces actuosus for High-Yield Nosiheptide Production

  • Qingling Wang
  • Dong Zhang
  • Yudong Li
  • Fuming Zhang
  • Cao Wang
  • Xinle Liang


Nosiheptide is one of the EU-approved sulfur-containing peptides in feed industry to inhibit the growth of the majority of Gram-positive bacteria. The main purpose of this study is directed to breed the high nosiheptide-producers by genome shuffling and ribosome engineering in Streptomyces actuosus AW7. The starting population for shuffling was generated by combining 60Coγ-irradiation with LiCl mutagenesis treatments on the spores. After four rounds of protoplast fusion exposed to streptomycin as adaptive pressure, a high-yield recombinant strain D92 was obtained. In a 10-L fermenter, nosiheptide production reached 1.54 g/L which was 9.20-fold compared to that of the parental strain. Hyphae development, metabolic process, and ribosomal protein S12 sequence were investigated to characterize the differentiation among the recombinants. Several mutations in S12 were believed to be responsible to streptomycin resistance in the tested strain. The results demonstrated that the combination of genome shuffling and ribosome engineering is an efficient approach to breed high-yield industrial strains.


Nosiheptide Genome shuffling Ribosome engineering Sequence difference Streptomyces actuosus 



The work was financially supported by the National Nature and Science Foundation of China (3117175)

Conflict of Interests

The authors declare that they have no conflict of interests.

Supplementary material

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Qingling Wang
    • 1
  • Dong Zhang
    • 1
  • Yudong Li
    • 1
  • Fuming Zhang
    • 2
  • Cao Wang
    • 1
    • 3
  • Xinle Liang
    • 1
  1. 1.Department of Biological EngineeringZhejiang Gongshang UniversityHangzhouChina
  2. 2.Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary StudiesRensselaer Polytechnic InstituteTroyUSA
  3. 3.Zhejiang University City CollegeHangzhouChina

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