Advertisement

Applied Biochemistry and Biotechnology

, Volume 173, Issue 5, pp 1236–1249 | Cite as

First Report of a Lipopeptide Biosurfactant from Thermophilic Bacterium Aneurinibacillus thermoaerophilus MK01 Newly Isolated from Municipal Landfill Site

  • Hakimeh Sharafi
  • Mahya Abdoli
  • Hamidreza Hajfarajollah
  • Nima Samie
  • Leila Alidoust
  • Habib Abbasi
  • Jamshid Fooladi
  • Hossein Shahbani Zahiri
  • Kambiz Akbari NoghabiEmail author
Article

Abstract

A biosurfactant-producing thermophile was isolated from the Kahrizak landfill of Tehran and identified as a bacterium belonging to the genus Aneurinibacillus. A thermostable lipopeptide-type biosurfactant was purified from the culture medium of this bacterium and showed stability in the temperature range of 20–90 °C and pH range of 5–10. The produced biosurfactant could reduce the surface tension of water from 72 to 43 mN/m with a CMC of 1.21 mg/mL. The strain growing at a temperature of 45 °C produces a substantial amount of 5 g/L of biosurfactant in the medium supplemented with sunflower oil as the sole carbon source. Response surface methodology was employed to optimize the biosurfactant production using sunflower oil, sodium nitrate, and yeast extract as variables. The optimization resulted in 6.75 g/L biosurfactant production, i.e., 35 % improved as compared to the unoptimized condition. Thin-layer chromatography, FTIR spectroscopy, 1H-NMR spectroscopy, and biochemical composition analysis confirmed the lipopeptide structure of the biosurfactant.

Keywords

Thermophiles Lipopeptide biosurfactant Aneurinibacillus thermoaerophilus MK01 Surface tension Thin-layer chromatography Response surface methodology 

Supplementary material

12010_2014_928_MOESM1_ESM.docx (45 kb)
Fig. S1 (DOCX 45 kb)

References

  1. 1.
    Rodrigues, L. R., Teixeira, J. A., van der Mei, H. C., & Oliveira, R. (2006). Colloids and Surfaces B: Biointerfaces, 49, 79–86.CrossRefGoogle Scholar
  2. 2.
    Ron, E. Z., & Rosenberg, E. (2002). Environmental Microbiology, 3, 229–236.CrossRefGoogle Scholar
  3. 3.
    Banat, I. M., Markkar, R. S., & Cameotra, S. S. (2000). Applied Microbiology and Biotechnology, 53, 495–508.CrossRefGoogle Scholar
  4. 4.
    Karanth, N. G. K., Deo, P. G., & Veenanadig, N. K. (1999). Current Science, 77, 116–123.Google Scholar
  5. 5.
    Cameotra, S. S., & Makkar, R. S. (2004). Current Opinion in Microbiology, 7, 262–266.CrossRefGoogle Scholar
  6. 6.
    Desai, J. D., & Banat, I. M. (1997). Microbiology and Molecular Biology Reviews, 61, 47–64.Google Scholar
  7. 7.
    Abbasi, H., Hamedi, M. M., Lotfabad, T. B., Zahiri, H. S., Sharafi, H., Masoomi, F., et al. (2012). Journal of Bioscience and Bioengineering, 113, 211–219.CrossRefGoogle Scholar
  8. 8.
    Joshi, S., Bharucha, C., Jha, S., Yadav, S., Nerurkar, A., & Desai, A. J. (2008). Bioresource Technology, 99, 195–199.CrossRefGoogle Scholar
  9. 9.
    Banat, I. M. (1993). Biotechnology Letters, 15, 591–594.CrossRefGoogle Scholar
  10. 10.
    Bharali, P., Das, S., Konwar, B. K., & Thakur, A. J. (2011). International Biodeterioration and Biodegradation, 65, 682–690.CrossRefGoogle Scholar
  11. 11.
    Mnif, S., Chamkha, M., Labat, M., & Sayadi, S. (2011). Journal of Applied Microbiology, 111, 525–536.CrossRefGoogle Scholar
  12. 12.
    Raj, A., Reddy, M. M. K., & Chandra, R. (2007). Journal of Chemical Technology and Biotechnology, 82, 399–406.CrossRefGoogle Scholar
  13. 13.
    King, J. D., Poon, K. K. H., Webb, N. A., Anderson, E. M., McNally, D. J., Brisson, J. R., et al. (2009). FEBS Journal, 276, 2686–2700.CrossRefGoogle Scholar
  14. 14.
    Masomian, M., Zaliha, R. N., Rahman, R. A., Salleh, A. B., & Basri, M. (2010). World Journal of Microbiology and Biotechnology, 26, 1693–1701.CrossRefGoogle Scholar
  15. 15.
    Murai, A., Tsujimoto, Y., Matsui, H., & Watanabe, K. (2004). Journal of Applied Microbiology, 96, 810–818.CrossRefGoogle Scholar
  16. 16.
    Schaffer, C., & Messner, P. (2001). Biochimie, 83, 591–599.CrossRefGoogle Scholar
  17. 17.
    Youssef, N. H., Duncan, K. E., Nagle, D. P., Savage, K. N., Knapp, R. M., & McInerney, M. J. (2004). Journal of Microbiological Methods, 56, 339–347.CrossRefGoogle Scholar
  18. 18.
    Chakravorty, S., Helb, D., Burday, M., Connell, N., & Alland, D. (2007). Journal of Microbiological Methods, 69, 330–339.CrossRefGoogle Scholar
  19. 19.
    Altschul, S. F., Gish, W., Miller, W., Myers, E. W., & Lipman, D. J. (1990). Journal of Molecular Biology, 215, 403–410.CrossRefGoogle Scholar
  20. 20.
    Tamura, K., Dudley, J., Nei, M., & Kumar, S. (2007). Molecular Biology and Evolution, 24, 1596–1599.CrossRefGoogle Scholar
  21. 21.
    Cooper, D. G., & Goldenberg, B. G. (1987). Applied and Environmental Microbiology, 53, 224–229.Google Scholar
  22. 22.
    Morikawa, M., Hirata, Y., & Imanaka, T. (2000). Biochimica et Biophysica Acta (BBA), 1488, 211–218.CrossRefGoogle Scholar
  23. 23.
    Das, P., Mukherjee, S., & Sen, R. (2009). Bioresource Technology, 100, 1015–1019.CrossRefGoogle Scholar
  24. 24.
    Yin, H., Qiang, J., Jia, Y., Ye, J., Peng, H., Qin, H., et al. (2009). Process Biochemistry, 44, 302–308.CrossRefGoogle Scholar
  25. 25.
    Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., & Smith, F. (1956). Analytical Chemistry, 28, 350–356.CrossRefGoogle Scholar
  26. 26.
    Folch, J. M., Leesm, M., & Stanlym, H. S. (1956). Journal of Biological Chemistry, 226, 497–509.Google Scholar
  27. 27.
    Bradford, M. M. (1976). Analytical Biochemistry, 72, 248–254.CrossRefGoogle Scholar
  28. 28.
    Montgomery, D. C. (1997). In design and analysis of experiments. New York: John Wiley and Sons.Google Scholar
  29. 29.
    Abbasi, H., Sharafi, H., Alidost, L., Bodagh, A., Zahiri, H. S., & Noghabi, K. A. (2013). Preparative Biochemistry & Biotechnology, 43, 398–414.CrossRefGoogle Scholar
  30. 30.
    Zhao, Z., Selvam, A., & Wong, J. W. (2011). Journal of Hazardous Materials, 190, 345–350.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Hakimeh Sharafi
    • 1
    • 2
  • Mahya Abdoli
    • 1
    • 3
  • Hamidreza Hajfarajollah
    • 4
  • Nima Samie
    • 1
  • Leila Alidoust
    • 1
  • Habib Abbasi
    • 5
  • Jamshid Fooladi
    • 2
  • Hossein Shahbani Zahiri
    • 1
  • Kambiz Akbari Noghabi
    • 1
    Email author
  1. 1.Division of Industrial & Environmental BiotechnologyNational Institute of Genetic Engineering and BiotechnologyTehranIran
  2. 2.Department of Microbiology, Faculty of SciencesAlzahra UniversityTehranIran
  3. 3.Faculty of AgriculturePayam Noor UniversityTehranIran
  4. 4.Chemistry and Chemical Engineering Research Center of IranTehranIran
  5. 5.Department of Chemical EngineeringJundi-Shapur University of TechnologyDezfulIran

Personalised recommendations