The Application of the Starfish Hatching Enzyme for the Improvement of Scar and Keloid Based on the Fibroblast-Populated Collagen Lattice
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Various bioactivities of the starfish hatching enzyme (HE) including collagen gel contraction, MMPs activity, hydroxyproline release, and gene regulation based on the fibroblast-populated collagen lattice (FPCL) in three-dimensional medium were investigated for the improvement of scar and keloid. The starfish HE significantly inhibited the collagen gel contraction over 2 days of culture. MMP-2 and MMP-9 activities were also identified by gelatin zymography and RT-PCR products with both HE and collagenase treatments, which resulted in the high amount of hydroxyproline release. The HE treatment on the FPCL significantly inhibited the fibroblast proliferation at 3 days of culture. The LPS-induced NO level and iNOS mRNA expression at low concentrations of HE presented a certain ability to inflammatory response. The COX-2 mRNA from the FPCL indicated no significant inflammation-mediated activity at 5 μg/mL of HE, whereas the cytokines of TNF-α and IL-1β were significantly higher than those of the control. Hence, the starfish hatching enzyme can regulate the fibroblast-populated collagen gel conditions by the contraction, MMP production, inflammatory gene expression, etc. Therefore, the starfish HE could be a potential cosmeceutical to heal the scar and keloid tissue.
KeywordsHatching enzyme Collagen gel contraction MMP Gene regulation
Tissue inhibitors metalloproteases
Fibroblast-populated collagen lattice
Inducible nitric oxide synthase
Tumor necrosis factor-alpha
Transfer growth factor
This research was supported by the Korea Sea Grant Program (GangWon Sea Grant) funded by the Ministry of Oceans and Fisheries in Korea.
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