Applied Biochemistry and Biotechnology

, Volume 173, Issue 4, pp 933–945 | Cite as

Protective Effects of Ginseng Leaf Extract Using Enzymatic Extraction Against Oxidative Damage of UVA-Irradiated Human Keratinocytes

  • Mi-Ryung Kim
  • Hyun-Sun Lee
  • Hyeon-Son Choi
  • Sun Young Kim
  • Yooheon Park
  • Hyung Joo SuhEmail author


UVA is responsible for numerous biological effects on the skin, including premature aging characterized by wrinkles, leathery texture, and mottled pigmentation. The objective of this study was evaluating the protective effect of ginseng leaf extract prepared by Ultraflo L on skin from photodamage. Anti-wrinkle effect of ginseng leaf extract with or without Ultraflo L treatment were tested on human keratinocyte cells (HaCaT) irradiated with ultraviolet (UV) A. Ginseng leaves inhibited ROS generation, GHS depletion, and expression of MMP-2 and MMP-9 induced by UVA irradiation. The glutathione (GSH) content of the cells was significantly increased by over 25 μg mL−1 of Ultraflo-treated extract (UTGL) as well as by over 100 μg mL−1 of nonenzyme-treated extract (NEGL) compared to control. UTGL and NEGL treatments significantly decreased expression of metalloproteinase (MMP)-2 and 9 compared with control, but inhibitory effects of two groups on expression of MMPs were not significantly different. Overall, ULtraflo L-treated ginseng leaves inhibited ROS generation, GHS depletion, and expression of MMP-2 and MMP-9 in UVA photodamaged HaCat cells. From these results, enzyme-treated ginseng leaf extract has advantages over untreated ginseng leaves and have potential as a skin protective ingredient against UVA-induced photodamage.


Panax ginseng leaves Ultraflo L Oxidative stress Phothodamage GSH MMPs 



This research was supported by a grant from RRI of KIAT, Korea (#B0009759).

Conflict of Interest

The authors declare that there are no conflicts of interest.


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Mi-Ryung Kim
    • 1
  • Hyun-Sun Lee
    • 2
  • Hyeon-Son Choi
    • 3
    • 5
  • Sun Young Kim
    • 4
  • Yooheon Park
    • 3
    • 5
  • Hyung Joo Suh
    • 3
    • 5
    Email author
  1. 1.OURHOME Co. Ltd Food R&D CenterSungnamRepublic of Korea
  2. 2.Agency for Korea Food ClusterFood Quality & Safety CenterGwacheonRepublic of Korea
  3. 3.Department of Food and NutritionKorea UniversitySeoulRepublic of Korea
  4. 4.Hongcheon Institute of Medicinal HerbsHongcheonRepublic of Korea
  5. 5.Department of Public Health Science, Graduate SchoolKorea UniversitySeoulRepublic of Korea

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