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Applied Biochemistry and Biotechnology

, Volume 172, Issue 8, pp 3736–3747 | Cite as

Biological Real-Time Reaction Calorimeter Studies for the Production of Penicillin G Acylase from Bacillus badius

  • Karthikeyan Rajendran
  • Sudharshan Sekar
  • Surianarayanan MahadevanEmail author
  • Bhuvanesh Kumar Shanmugam
  • Rajendhran Jeyaprakash
  • Gunasekaran Paramasamy
  • Asit Baran Mandal
Article

Abstract

Penicillin G acylase (PGA) is a commercially important enzyme that cleaves penicillin G to 6-amino penicillanic acid (6-APA) and phenyl acetic acid (PAA). The strain Bacillus badius has been identified as potential producer of PGA. A detailed calorimetric investigation on PGA production was carried out to enable generation of thermokinetic data possible for commercial application. Reaction calorimetric studies coupled with respirometric studies suggested that enzyme activity of the species B. badius was calorimetrically traceable. Three phases of growth were distinctly noticeable in the metabolic heat-time curve. Increase in enzymatic activity with restricted growth confirmed intracellular nature of the production process. The estimated heat yields due to biomass growth, 10.026 kJ/g, substrate consumption 22.761 kJ/g, and oxygen uptake 383 ± 10 kJ/mol helped to understand the energetic of the organism under study. Low oxycalorific coefficient confirmed the existence of fermentation-coupled metabolism of B. badius.

Keywords

Penicillin G acylase (PGA) 6-Amino penicillanic acid (6-APA) Bacillus badius (B. badiusRespirometry Oxycalorific coefficient 

Nomenclature

Symbols

A

Heat transfer area (m2)

Q

Heat generated (kJ)

Qmet (t)

Cumulative metabolic heat (kJ/L)

OUR

Oxygen uptake rate (mg/L min)

q

Heat evolution rate (W)

qa

Heat flow of the acid or base addition (W)

qac

Heat accumulation in the bulk (W)

qb1

Baseline heat (W)

\( {q}_{{\mathrm{CO}}_2} \)

Heat flow of the CO2 vaporization (W)

qe

Heat flow to the environment through the non-jacketed part of the reactor (W)

qf

Heat flow of the feed (W)

qg

Heat flow induced by aeration (W)

qj

Heat flow through the reactor wall to the jacket oil (W)

qs

Heat flow due to the stirring power (W)

qr

Heat generated by the reaction (W)

Tj

Temperature of jacket oil (°C)

Tr

Temperature of reactor contents (°C)

U

Global heat transfer coefficient (W/m2K)

Vvm

Volume of air per volume of medium per minute (L/L min)

YQ/X

Heat yield coefficient with respect to biomass (kJ/g)

YX/S

Biomass yield coefficient with respect to substrate (g/g)

YQ/O

Heat generated due to oxygen consumed (kJ/mol)

YQ/S

Heat yield due to substrate depletion (kJ/g)

YX/O

Biomass yield due to oxygen consumption (g/g mol)

Subscript

O

Oxygen

S

Substrate

X

Biomass

Notes

Acknowledgements

One of the authors (RK) wishes to acknowledge the Principal of Mepco Schlenk Engineering College for kind permission to publish the paper. The authors wish to express their gratitude to Prof. NR Rajagopal for encouragement.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Karthikeyan Rajendran
    • 1
  • Sudharshan Sekar
    • 2
  • Surianarayanan Mahadevan
    • 2
    Email author
  • Bhuvanesh Kumar Shanmugam
    • 2
  • Rajendhran Jeyaprakash
    • 3
  • Gunasekaran Paramasamy
    • 3
  • Asit Baran Mandal
    • 2
  1. 1.Department of BiotechnologyMepco Schlenk Engineering CollegeSivakasiIndia
  2. 2.Chemical Engineering DepartmentCentral Leather Research Institute (CLRI)ChennaiIndia
  3. 3.Centre for Excellence in Genomic Sciences, School of Biological SciencesMadurai Kamaraj UniversityMaduraiIndia

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