Applied Biochemistry and Biotechnology

, Volume 172, Issue 7, pp 3529–3537 | Cite as

A β-Secretase (BACE1)-inhibiting C-Methylrotenoid Induced by Yeast Elicitation in Abronia nana Suspension Cultures

  • Sang-In Kim
  • Eun-Ju Yang
  • Se-Hoon Park
  • Chang-Kil Kim
  • Kyung-Sik Song
Article
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Accepted:

Abstract

Suspension cultures of Abronia nana were established to produce C-methylisoflavones. Treatment of the A. nana cultures with yeast elicitor induced boeravinone E (1), with maximum induction at 24 h after elicitor treatment. Of the biotic and abiotic elicitors tested, yeast extract gave the strongest induction of 1. The IC50 value of 1 against β-secretase (β-amyloid cleaving enzyme-1) was 5.57 μM. Other proteases such as trypsin, chymotrypsin, and elastase were not inhibited by concentrations up to 1.0 mM, indicating that inhibition of β-secretase was specific. 1 was noncompetitive in Dixon plot, and Ki value was 3.79 μM.

Keywords

Abronia nana Suspension cultures C-methylrotenoid Boeravinone E β-secretase inhibitor Yeast elicitation 
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Notes

Acknowledgments

This work was supported by a National Research Foundation of Korea grant funded by the Korean Government (NRF-2012R1A1A2039509). The authors also thank Kyungpook National University Research Fund, 2012, for partial financial support.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Sang-In Kim
    • 1
  • Eun-Ju Yang
    • 1
  • Se-Hoon Park
    • 1
  • Chang-Kil Kim
    • 1
    • 2
  • Kyung-Sik Song
    • 1
  1. 1.Research Institute of Pharmaceutical Sciences, College of PharmacyKyungpook National UniversityDaeguRepublic of Korea
  2. 2.Department of Horticultural Science, College of Agriculture and Life SciencesKyungpook National UniversityDaeguRepublic of Korea

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