Applied Biochemistry and Biotechnology

, Volume 172, Issue 4, pp 2266–2275 | Cite as

Aqueous Extract of Crataegus azarolus Protects Against DNA Damage in Human Lymphoblast Cell K562 and Enhances Antioxidant Activity

  • Nadia Mustapha
  • Inès Bouhlel
  • Fadwa Chaabane
  • Imèn Mokdad Bzéouich
  • Kamel Ghedira
  • Thierry Hennebelle
  • Leila Chekir-GhediraEmail author


The present study was carried out to characterize the cellular antioxidant effect of the aqueous extract of Crataegus azarolus and its antigenotoxic potential using human myelogenous cells, K562. The antioxidant capacity of this extract was evaluated by determining its cellular antioxidant activity (CAA) in K562 cells. Also, preceding antigenotoxicity assessment, its eventual genotoxicity property was investigated by evaluating its capacity to induce the DNA degradation of treated cell nuclei. As no genotoxicity was detected at different exposure times, its ability to protect cell DNA against H2O2 oxidative effect was investigated, using the “comet assay.” It appears that 800 μg/mL of extract inhibited the genotoxicity induced by H2O2 with a rate of 41.30 %, after 4 h of incubation. In addition, this extract revealed a significant cellular antioxidant capacity against the reactive oxygen species in K562 cells.


Crataegus azarolus Antigenotoxic activity Comet assay Cellular antioxidant activity K562 


Conflict of Interest

No conflict of interest is declared.


  1. 1.
    Barnes, J., Anderson, L. A., & Phillipson, J. D. (2002). Herbal Medicines 2002. London: Pharmaceutical Press.Google Scholar
  2. 2.
    Bahorun, T., Aumjaud, E., Ramphul, H., Rycha, M., Luximon-Ramma, A., Trotin, F., et al. (2003). Phenolic constituents and antioxidant capacities of Crataegus monogyna (Hawthorn) callus extracts. Nahrung, 47, 191–198.CrossRefGoogle Scholar
  3. 3.
    Chang, Q., Zuo, Z., Harrison, F., & Chow, M. S. (2002). Hawthorn. Journal of Clinical Pharmacology, 42, 605–612.CrossRefGoogle Scholar
  4. 4.
    Pottier-Alapetite, G. (1979). Flore de la Tunisia: Angiospermes, Dicotyledones, Apetales, Dialypetales. Tunis: Ministère de L'Enseignement Supérieur et de la Recherche Scientifique et Ministère de l'Agriculture.Google Scholar
  5. 5.
    Boukef, M.K. (1986). Les plantes dans la médecine traditionnelle tunisienne, médecine traditionnelle et pharmacopée. Paris: Agence de Coopération Culturelle et Technique.Google Scholar
  6. 6.
    Aruoma, O. I. (1999). Antioxidant action of plant foods: use of oxidative DNA damage as a tool for studying antioxidant efficacy. Free Radical Research, 30, 419–427.CrossRefGoogle Scholar
  7. 7.
    Edwards, J. E., Brown, P. N., Talent, N., Dickinson, T. A., & Shipley, P. R. (2012). A review of the chemistry of the genus Crataegus. Phytochemistry, 79, 5–26.CrossRefGoogle Scholar
  8. 8.
    Scassellati-Sforzolini, G., Villarini, M., Moretti, M., Marcarelli, M., Pasquini, R., Fatigoni, C., et al. (1999). Antigenotoxic properties of Terminalia arjuna bark extracts. Journal of Environmental Pathology, Toxicology and Oncology, 18, 119–125.Google Scholar
  9. 9.
    Collins, A. R., Dusinska, M., Gedik, C. M., & Stetina, R. (1996). Oxidative damage to DNA: do we have a reliable biomarker? Environmental Health Perspectives, 104, 465–469.Google Scholar
  10. 10.
    Wolfe, K. L., & Liu, R. H. (2007). Cellular antioxidant activity (CAA) assay for assessing antioxidants, foods, and dietary supplements. Journal of Agricultural and Food Chemistry, 55, 8896–8907.CrossRefGoogle Scholar
  11. 11.
    Limem-Ben Amor, I., Skandrani, I., Boubaker, J., Ben Sghaier, M., Neffati, M. A., Bhouri, W., et al. (2009). Investigation of biological activity of polar extracts isolated from Phlomis crinita Cav ssp. mauritanica Munby. Drug and Chemical Toxicology, 32, 38–46.CrossRefGoogle Scholar
  12. 12.
    Skandrani, I., Boubaker, J., Bouhlel, I., Limem, I., Ghedira, K., & Chekir-Ghedira, L. (2010). Leaf and root extracts of Moricandia arvensis protect against DNA damage in human lymphoblast cell K562 and enhance antioxidant activity. Environmental Toxicology and Pharmacology, 30, 61–67.CrossRefGoogle Scholar
  13. 13.
    Ben Sghaier, M., Harizi, H., Louhichi, T., Krifa, M., Ghedira, K., & Chekir-Ghedira, L. (2011). Anti-inflammatory and antiulcerogenic activities of leaf extracts and sesquiterpene from Teucrium ramosissimum (Lamiaceae). Immunopharmacology and Immunotoxicology, 33, 656–662.CrossRefGoogle Scholar
  14. 14.
    Krifa, M., Bouhlel, I., Ghedira-Chekir, L., & Ghedira, K. (2013). Immunomodulatory and cellular anti-oxidant activities of an aqueous extract of Limoniastrum guyonianum gall. Journal of Ethnopharmacology, 146, 243–249.CrossRefGoogle Scholar
  15. 15.
    Wang, H., Du, Y. J., & Song, H. C. (2010). α-Glucosidase and α-amylase inhibitory activities of guava leaves. Food Chemistry, 123, 6–13.CrossRefGoogle Scholar
  16. 16.
    Piao, M. J., Kang, K. A., Zhang, R., Ko, D. O., Wang, Z. H., You, H. J., et al. (2008). Hyperoside prevents oxidative damage induced by hydrogen peroxide in lung fibroblast cells via an antioxidant effect. Biochimica et Biophysica Acta, 1780, 1448–1457.CrossRefGoogle Scholar
  17. 17.
    Zou, Y., Lu, Y., & Wei, D. (2004). Antioxidant activity of a flavonoid-rich extract of Hypericum perforatum L. in vitro. Journal of Agricultural and Food Chemistry, 52, 5032–5039.CrossRefGoogle Scholar
  18. 18.
    Kaur, P., Kaur, S., Kumar, N., Singh, B., & Kumar, S. (2009). Evaluation of antigenotoxic activity of isoliquiritin apioside from Glycyrrhiza glabra L. Toxicology in Vitro, 23, 680–686.CrossRefGoogle Scholar
  19. 19.
    Kumar, A., & Chattopadhyay, S. (2006). DNA damage protecting activity and antioxidant potential of pudina extract. Food Chemistry, 100, 1377–1384.CrossRefGoogle Scholar
  20. 20.
    Kun-Young, P., Geun-Ok, J., Kyung-Tae, L., Jongwon, C., Moo-Young, C., Gab-Tar, K., et al. (2004). Antimutagenic activity of flavonoids from the heartwood of Rhus verniciflua. Journal of Ethnopharmacology, 90, 73–79.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Nadia Mustapha
    • 1
    • 2
  • Inès Bouhlel
    • 1
    • 2
  • Fadwa Chaabane
    • 1
    • 2
  • Imèn Mokdad Bzéouich
    • 1
    • 2
  • Kamel Ghedira
    • 2
  • Thierry Hennebelle
    • 3
  • Leila Chekir-Ghedira
    • 1
    • 2
    Email author
  1. 1.Laboratoire de Biologie Cellulaire et Moléculaire, Faculté de Médecine DentaireUniversité de MonastirMonastirTunisia
  2. 2.Unité de Substances Naturelles Bioactives et Biotechnologie UR12ES12, Faculté de Pharmacie de MonastirUniversité de MonastirMonastirTunisia
  3. 3.Laboratoire de Pharmacognosie, E.A. 4481, Faculté de PharmacieUniversité de Lille 2Lille CEDEXFrance

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