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Applied Biochemistry and Biotechnology

, Volume 172, Issue 4, pp 2266–2275 | Cite as

Aqueous Extract of Crataegus azarolus Protects Against DNA Damage in Human Lymphoblast Cell K562 and Enhances Antioxidant Activity

  • Nadia Mustapha
  • Inès Bouhlel
  • Fadwa Chaabane
  • Imèn Mokdad Bzéouich
  • Kamel Ghedira
  • Thierry Hennebelle
  • Leila Chekir-GhediraEmail author
Article

Abstract

The present study was carried out to characterize the cellular antioxidant effect of the aqueous extract of Crataegus azarolus and its antigenotoxic potential using human myelogenous cells, K562. The antioxidant capacity of this extract was evaluated by determining its cellular antioxidant activity (CAA) in K562 cells. Also, preceding antigenotoxicity assessment, its eventual genotoxicity property was investigated by evaluating its capacity to induce the DNA degradation of treated cell nuclei. As no genotoxicity was detected at different exposure times, its ability to protect cell DNA against H2O2 oxidative effect was investigated, using the “comet assay.” It appears that 800 μg/mL of extract inhibited the genotoxicity induced by H2O2 with a rate of 41.30 %, after 4 h of incubation. In addition, this extract revealed a significant cellular antioxidant capacity against the reactive oxygen species in K562 cells.

Keywords

Crataegus azarolus Antigenotoxic activity Comet assay Cellular antioxidant activity K562 

Notes

Conflict of Interest

No conflict of interest is declared.

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Nadia Mustapha
    • 1
    • 2
  • Inès Bouhlel
    • 1
    • 2
  • Fadwa Chaabane
    • 1
    • 2
  • Imèn Mokdad Bzéouich
    • 1
    • 2
  • Kamel Ghedira
    • 2
  • Thierry Hennebelle
    • 3
  • Leila Chekir-Ghedira
    • 1
    • 2
    Email author
  1. 1.Laboratoire de Biologie Cellulaire et Moléculaire, Faculté de Médecine DentaireUniversité de MonastirMonastirTunisia
  2. 2.Unité de Substances Naturelles Bioactives et Biotechnologie UR12ES12, Faculté de Pharmacie de MonastirUniversité de MonastirMonastirTunisia
  3. 3.Laboratoire de Pharmacognosie, E.A. 4481, Faculté de PharmacieUniversité de Lille 2Lille CEDEXFrance

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