Applied Biochemistry and Biotechnology

, Volume 172, Issue 4, pp 1790–1806 | Cite as

Investigations on the Interactions of λPhage-Derived Peptides Against the SrtA Mechanism in Bacillus anthracis

  • Chandrabose Selvaraj
  • Poonam Singh
  • Sanjeev Kumar Singh
Article
First Online:
Received:
Accepted:

Abstract

Bacillus anthracis is a well-known bioweapon pathogen, which coordinates the expression of its virulence factors in response to a specific environmental signal by its protein architecture. Absences of sortase signal functioning may fail to assemble the surface linked proteins and so B. anthracis cannot sustain an infection with host cells. Targeting the signaling mechanism of B. anthracis can be achieved by inhibition of SrtA enzyme through λphage-derived plyG. The lysin enzyme plyG is experimentally proven as bacteriolytic agent, specifically kill's B. anthracis by inhibiting the SrtA. Here, we have screened the peptides from λphage lysin, and these peptides are having the ability as LPXTG competitive inhibitors. In comparison to the activator peptide LPXTG binding motif, λphage lysin based inhibitor peptides are having much supremacy towards binding of SrtA. Finally, peptide structures extracted from PlyG are free from toxic, allergic abilities and also have the ability to terminate the signal transduction mechanism in B. anthracis.

Keywords

Activator LPXTG Peptide PlyG Peptidomimetic SrtA 
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Notes

Acknowledgments

The authors thankfully acknowledge the CSIR for research funding and fellowship grants (Ref. No: 37(1491)/11/EMR-II). One of the authors Chandrabose Selvaraj gratefully acknowledges CSIR for the Senior Research Fellowship (SRF). The authors thank anonymous referees for the valuable suggestion on this research work.

Conflict of Interests

None

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Chandrabose Selvaraj
    • 1
  • Poonam Singh
    • 2
  • Sanjeev Kumar Singh
    • 1
  1. 1.Computer-Aided Drug Design and Molecular Modeling Lab, Department of BioinformaticsAlagappa UniversityKaraikudiIndia
  2. 2.Toxicology DivisionCentral Drug Research InstituteLucknowIndia

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