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Applied Biochemistry and Biotechnology

, Volume 172, Issue 3, pp 1347–1357 | Cite as

Designing of an Intensification Process for Biosynthesis and Recovery of Menaquinone-7

  • Aydin BerenjianEmail author
  • Raja Mahanama
  • Andrea Talbot
  • Hubert Regtop
  • John Kavanagh
  • Fariba Dehghani
Article

Abstract

A nutritional food rich in menaquinone-7 has a potential in preventing osteoporosis and cardiovascular diseases. The static fermentation of Bacillus subtilis natto is widely regarded as an optimum process for menaquinone-7 production. The major issues for the bulk production of menaquinone-7 are the low fermentation yield, biofilm formation and the use of organic solvents for the vitamin extraction. In this study, we demonstrate that the dynamic fermentation involving high stirring and aeration rates enhances the yield of fermentation process significantly compared to static system. The menaquinone-7 concentration of 226 mg/L was produced at 1,000 rpm, 5 vvm, 40 °C after 5 days of fermentation. This concentration is 70-fold higher than commercially available food products such as natto. Additionally, it was found that more than 80 % of menaquinone-7 was recovered in situ in the vegetable oil that was gradually added to the system as an anti-foaming agent. The intensification process developed in this study has a capacity to produce an oil rich in menaquinone-7 in one step and eliminate the use of organic solvents for recovery of this compound. This oil can, therefore, be used for the preparation of broad range of supplementary and dietary food products rich in menaquinone-7 to reduce the risk of osteoporotic fractures and cardiovascular diseases.

Keywords

Menaquinone-7 Stirring Aeration Bacillus subtilis natto Anti-foam Extraction Process intensification 

Notes

Acknowledgments

The authors acknowledge the financial support by the Australian Research Council (LP100100347) and Agricure Scientific Organics. RM also acknowledges the financial support of Henry Bertie and Florence Mabel Gritton scholarship.

Conflict of Interest

The authors report no conflicts of interest.

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Aydin Berenjian
    • 1
    Email author
  • Raja Mahanama
    • 1
  • Andrea Talbot
    • 2
  • Hubert Regtop
    • 2
  • John Kavanagh
    • 1
  • Fariba Dehghani
    • 1
  1. 1.School of Chemical and Biomolecular EngineeringThe University of SydneySydneyAustralia
  2. 2.Agricure Scientific OrganicsBraemarAustralia

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