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Applied Biochemistry and Biotechnology

, Volume 172, Issue 2, pp 1098–1105 | Cite as

Antifungal Activity of Microgramma vacciniifolia Rhizome Lectin on Genetically Distinct Fusarium oxysporum f. sp. lycopersici Races

  • Lidiane Pereira de Albuquerque
  • Giselly Maria de Sá Santana
  • Thiago Henrique Napoleão
  • Luana Cassandra Breitenbach Barroso Coelho
  • Márcia Vanusa da Silva
  • Patrícia Maria Guedes PaivaEmail author
Article

Abstract

Fusarium oxysporum f. sp. lycopersici races 1, 2, and 3 deteriorate tomato crops since they cause a vascular wilt. Lectins are carbohydrate-binding proteins with hemagglutinating and antifungal activities. This work reports that Microgramma vacciniifolia rhizome lectin (MvRL) inhibits F. oxysporum f. sp. lycopersici race 3 growth (61 %) more intensely than of races 1 (55 %) and 2 (45 %). The hemagglutinating activity of MvRL was inhibited by glycoprotein preparations from mycelia of races 1, 2, and 3, and these data indicate that lectin carbohydrate-binding sites recognized glycosylated molecules from races. Inter-simple sequence repeat (ISSR) marker system showed that race 3 is genetically distinct from races 1 and 2, and thus the highest sensitiveness of F. oxysporum f. sp. lycopersici race 3 to MvRL may be due to molecular characteristics of this race.

Keywords

Microgramma vacciniifolia Rhizome lectin Antifungal activity Fusarium oxysporum f. sp. lycopersici ISSR 

Notes

Acknowledgments

The authors express their gratitude to the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for research grants and fellowships (LCBBC and PMGP), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (FACEPE), and Ministério da Ciência, Tecnologia e Inovação (MCTI) for research grants. L.P. Albuquerque would like to thank CAPES and the Brazilian Ministry of Education (Programa de Reestruturação e Expansão das Universidades Federais - REUNI) for graduate scholarship. G.M.S. Santana would like to thank FACEPE for graduate scholarship. The authors are also deeply grateful to Maria Barbosa Reis da Silva for the technical assistance and Felix Nonnenmacher for English editing.

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Lidiane Pereira de Albuquerque
    • 1
  • Giselly Maria de Sá Santana
    • 1
  • Thiago Henrique Napoleão
    • 1
  • Luana Cassandra Breitenbach Barroso Coelho
    • 1
  • Márcia Vanusa da Silva
    • 1
  • Patrícia Maria Guedes Paiva
    • 1
    Email author
  1. 1.Departamento de Bioquímica, Centro de Ciências BiológicasUniversidade Federal de PernambucoRecifeBrazil

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