Applied Biochemistry and Biotechnology

, Volume 172, Issue 2, pp 1121–1130 | Cite as

Cultivation of Chlorella vulgaris in Dairy Wastewater Pretreated by UV Irradiation and Sodium Hypochlorite

  • Lei Qin
  • Qing Shu
  • Zhongming Wang
  • Changhua Shang
  • Shunni Zhu
  • Jingliang Xu
  • Rongqing Li
  • Liandong Zhu
  • Zhenhong YuanEmail author


There is potential in the utilization of microalgae for the purification of wastewater as well as recycling the resource in the wastewater to produce biodiesel. The large-scale cultivation of microalgae requires pretreatment of the wastewater to eliminate bacteria and protozoa. This procedure is costly and complex. In this study, two methods of pretreatment, UV irradiation, and sodium hypochlorite (NaClO), in various doses and concentrations, were tested in the dairy wastewater. Combining the efficiency of biodiesel production, we proposed to treat the dairy wastewater with NaClO in the concentration of 30 ppm. In this condition, The highest biomass productivity and lipid productivity of Chlorella vulgaris reached 0.450 g L−1 day−1 and 51 mg L−1 day−1 after a 4-day cultivation in the dairy wastewater, respectively.


Dairy Wastewater Chlorella vulgaris UV Irradiation Sodium Hypochlorite Pretreatment Large-Scale Cultivation 



The authors are grateful for the support of the National Key Technology R&D Program of the 12th Five-year Plan of China (Grant No. 2011BAD14B03), the National High Technology Research and Development Program of China (Grant No. 2013AA065803), the National Natural Science Foundation of China (Grant No. 31100189), the Open Project of Jiangsu Key Laboratory for Biomass-based Energy and Enzyme Technology (Grant No. JSBEET1223), and the State Key Laboratory of Freshwater Ecology and Biotechnology (Grant No. 2013FB14).


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Lei Qin
    • 1
  • Qing Shu
    • 1
  • Zhongming Wang
    • 1
  • Changhua Shang
    • 1
  • Shunni Zhu
    • 1
  • Jingliang Xu
    • 1
  • Rongqing Li
    • 2
  • Liandong Zhu
    • 3
  • Zhenhong Yuan
    • 1
    Email author
  1. 1.Guangzhou Institute of Energy Conversion, Key Laboratory of Renewable EnergyChinese Academy of SciencesGuangzhouChina
  2. 2.Jiangsu Key Laboratory for Biomass-based Energy and Enzyme TechnologyHuaiyin Normal UniversityHuai’anChina
  3. 3.Faculty of TechnologyUniversity of Vaasa and Vaasa Energy InstituteVaasaFinland

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