Applied Biochemistry and Biotechnology

, Volume 171, Issue 6, pp 1362–1375 | Cite as

Application of Lipopeptide Biosurfactant Isolated from a Halophile: Bacillus tequilensis CH for Inhibition of Biofilm

  • Arun Kumar Pradhan
  • Nilotpala Pradhan
  • Gangotri Mall
  • Himadri Tanaya Panda
  • Lala Behari Sukla
  • Prasanna Kumar Panda
  • Barada Kanta Mishra


Biosurfactants are amphiphilic molecules having hydrophobic and hydrophilic moieties produced by various microorganisms. These molecules trigger the reduction of surface tension or interfacial tension in liquids. A biosurfactant-producing halophile was isolated from Lake Chilika, a brackish water lake of Odisha, India (19°41′39″N, 85°18′24″E). The halophile was identified as Bacillus tequilensis CH by biochemical tests and 16S rRNA gene sequencing and assigned accession no. KC851857 by GenBank. The biosurfactant produced by B. tequilensis CH was partially characterized as a lipopeptide using thin-layer chromatography, Fourier transform infrared spectroscopy, and nuclear magnetic resonance techniques. The minimum effective concentration of a biosurfactant for inhibition of pathogenic biofilm (Escherichia coli and Streptococcus mutans) on hydrophilic and hydrophobic surfaces was found to be 50 μg ml−1. This finding has potential for a variety of applications.


Halophiles Bacillus tequilensis CH Lipopeptides Biosurfactants Biofilm 



One of the authors (AKP) would like to express his sincere thanks to the Council of Scientific and Industrial Research (CSIR), India, for the award of Senior Research Fellowship. The authors are thankful to Eepsita Priyadarshini and Jacintha Esther for editorial assistance in manuscript preparation.


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Arun Kumar Pradhan
    • 1
    • 2
  • Nilotpala Pradhan
    • 1
    • 2
  • Gangotri Mall
    • 2
  • Himadri Tanaya Panda
    • 2
  • Lala Behari Sukla
    • 2
  • Prasanna Kumar Panda
    • 2
  • Barada Kanta Mishra
    • 3
  1. 1.Academy of Scientific and Innovative ResearchCSIR–Institute of Minerals and Materials TechnologyBhubaneswarIndia
  2. 2.Bioresources Engineering DepartmentCSIR–Institute of Minerals and Materials TechnologyBhubaneswarIndia
  3. 3.CSIR–Institute of Minerals and Materials TechnologyBhubaneswarIndia

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