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Applied Biochemistry and Biotechnology

, Volume 170, Issue 5, pp 1119–1126 | Cite as

Three-Phase Partitioning as a Rapid and Easy Method for the Purification and Recovery of Catalase from Sweet Potato Tubers (Solanum tuberosum)

  • Yonca Avcı DumanEmail author
  • Erdem Kaya
Article

Abstract

Three-phase partitioning (TPP) was used to purify and recover catalase from potato crude extract. The method consists of ammonium sulfate saturation, t-butanol addition, and adjustment of pH, respectively. The best catalase recovery (262 %) and 14.1-fold purification were seen in the interfacial phase in the presence of 40 % (w/v) ammonium sulfate saturation with 1.0:1.0 crude extract/t-butanol ratio (v/v) at pH 7 in a single step. The sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the enzyme showed comparatively purification and protein molecular weight was nearly found to be 56 kDa. This study shows that TPP is a simple, economical, and quick method for the recovering of catalase and can be used for the purification process.

Keywords

Three-phase partitioning Recovery Catalase Purification 

Notes

Acknowledgements

This study was performed within the project with number 2012/057 which was supported by Kocaeli Univresity Scientific Researches Unit.

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.Section of Biochemistry, Department of Chemistry, Faculty of Arts and SciencesKocaeli UniversityİzmitTurkey

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