Computational Approach for Identification of Anopheles gambiae miRNA Involved in Modulation of Host Immune Response
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MicroRNAs (miRNAs) are small, noncoding RNAs that play key roles in regulating gene expression in animals, plants, and viruses, which involves in biological processes including development, cancer, immunity, and host–microorganism interactions. In this present study, we have used the computational approach to identify potent miRNAs involved in Anopheles gambiae immune response. Analysis of 217,261 A. gambiae ESTs and further study of RNA folding revealed six new miRNAs. The minimum free energy of the predicted miRNAs ranged from −27.2 to −62.63 kcal/mol with an average of −49.38 kcal/mol. While its A + U % ranges from 50 to 65 % with an average value of 57.37 %. Phylogenetic analysis of the predicted miRNAs revealed that aga-miR-277 was evolutionary highly conserved with more similarity with other mosquito species. Observing further the target identification of the predicted miRNA, it was noticed that the aga-miR-2304 and aga-miR-2390 are involved in modulation of immune response by targeting the gene encoding suppressin and protein prophenoloxidase. Further detailed studies of these miRNAs will help in revealing its function in modulation of A. gambiae immune response with respect to its parasite.
KeywordsmiRNA A. gambiae Immune response miRNA target EST sequence
All the authors are thankful to the Pondicherry Centre for Biological centre (PCBS) for providing the necessary facility to carry out the work. Financial support as startup loan from State Bank of India (RASMECC), Pondicherry, India to establish the institute is also gratefully acknowledged.
- 6.Ambros, V., & Lee, R. C. (2004). Identification of microRNAs and other tiny noncoding RNAs by cDNA cloning. Methods Mol Biol, 265, 131–158.Google Scholar
- 8.Xie, J., Techritz, S., Haebel, S., Horn, A., Neitzel, H., Klose, J., et al. (2005). A two-dimensional electrophoretic map of human mitochondrial proteins from immortalized lymphoblastoid cell lines: a prerequisite to study mitochondrial disorders in patients. Proteomics, 5(11), 2981–2999.CrossRefGoogle Scholar
- 17.Altschul, S. F., Gish, W., Miller, W., Myers, E. W., & Lipman, D. J. (1990). Basic local alignment search tool. J Mol Biol, 215(3), 403–410.Google Scholar
- 30.Chatterjee, R., & Chaudhuri, K. (2006). An approach for the identification of microRNA with an application to Anopheles gambiae. Acta Biochim Pol, 53(2), 303–309.Google Scholar