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Applied Biochemistry and Biotechnology

, Volume 170, Issue 2, pp 281–291 | Cite as

Computational Approach for Identification of Anopheles gambiae miRNA Involved in Modulation of Host Immune Response

  • Krishnaraj Thirugnanasambantham
  • Villianur Ibrahim Hairul-Islam
  • Subramanian Saravanan
  • Subramaniyan Subasri
  • Ariraman Subastri
Article

Abstract

MicroRNAs (miRNAs) are small, noncoding RNAs that play key roles in regulating gene expression in animals, plants, and viruses, which involves in biological processes including development, cancer, immunity, and host–microorganism interactions. In this present study, we have used the computational approach to identify potent miRNAs involved in Anopheles gambiae immune response. Analysis of 217,261 A. gambiae ESTs and further study of RNA folding revealed six new miRNAs. The minimum free energy of the predicted miRNAs ranged from −27.2 to −62.63 kcal/mol with an average of −49.38 kcal/mol. While its A + U % ranges from 50 to 65 % with an average value of 57.37 %. Phylogenetic analysis of the predicted miRNAs revealed that aga-miR-277 was evolutionary highly conserved with more similarity with other mosquito species. Observing further the target identification of the predicted miRNA, it was noticed that the aga-miR-2304 and aga-miR-2390 are involved in modulation of immune response by targeting the gene encoding suppressin and protein prophenoloxidase. Further detailed studies of these miRNAs will help in revealing its function in modulation of A. gambiae immune response with respect to its parasite.

Keywords

miRNA A. gambiae Immune response miRNA target EST sequence 

Notes

Acknowledgments

All the authors are thankful to the Pondicherry Centre for Biological centre (PCBS) for providing the necessary facility to carry out the work. Financial support as startup loan from State Bank of India (RASMECC), Pondicherry, India to establish the institute is also gratefully acknowledged.

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Krishnaraj Thirugnanasambantham
    • 1
  • Villianur Ibrahim Hairul-Islam
    • 1
  • Subramanian Saravanan
    • 2
  • Subramaniyan Subasri
    • 1
    • 3
  • Ariraman Subastri
    • 1
    • 3
  1. 1.Pondicherry Centre for Biological Sciences, Jawahar NagarPondicherryIndia
  2. 2.Division of Ethnopharmacology, Entomology Research InstituteLoyola CollegeChennaiIndia
  3. 3.Department of Biochemistry and Molecular biologySchool of Life Sciences, Pondicherry UniversityPondicherryIndia

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