Applied Biochemistry and Biotechnology

, Volume 169, Issue 6, pp 1895–1909 | Cite as

Optimized Fed-Batch Fermentation of Scheffersomyces stipitis for Efficient Production of Ethanol from Hexoses and Pentoses

Article
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Abstract

Scheffersomyces stipitis was cultivated in an optimized, controlled fed-batch fermentation for production of ethanol from glucose–xylose mixture. Effect of feed medium composition was investigated on sugar utilization and ethanol production. Studying influence of specific cell growth rate on ethanol fermentation performance showed the carbon flow towards ethanol synthesis decreased with increasing cell growth rate. The optimum specific growth rate to achieve efficient ethanol production performance from a glucose-xylose mixture existed at 0.1 h−1. With these optimized feed medium and cell growth rate, a kinetic model has been utilized to avoid overflow metabolism as well as to ensure a balanced feeding of nutrient substrate in fed-batch system. Fed-batch culture with feeding profile designed based on the model resulted in high titer, yield, and productivity of ethanol compared with batch cultures. The maximal ethanol concentration was 40.7 g/L. The yield and productivity of ethanol production in the optimized fed-batch culture was 1.3 and 2 times higher than those in batch culture. Thus, higher efficiency ethanol production was achieved in this study through fed-batch process optimization. This strategy may contribute to an improvement of ethanol fermentation from lignocellulosic biomass by S. stipitis on the industrial scale.

Keywords

Ethanol fermentation Scheffersomyces stipitis Fed-batch Kinetic model Feed medium composition Controlled specific growth rate 
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Notes

Acknowledgment

This work has been supported by Thailand Research Fund (TRF) Grant for New Researcher.

References

  1. 1.
    Agbogbo, F. K., & Coward-Kelly, G. (2008). Cellulosic ethanol production using the naturally occurring xylose-fermenting yeast, Pichia stipitis. Biotechnology Letters, 30, 1515–1524.CrossRefGoogle Scholar
  2. 2.
    Agbogbo, F. K., Coward-Kelly, G., Torry-Smith, M., Wenger, K., & Jeffries, T. W. (2007). The effect of initial cell concentration on xylose fermentation by Pichia stipitis. Applied Biochemistry and Biotechnology, 137, 653–662.CrossRefGoogle Scholar
  3. 3.
    Andersson, L., Stranberg, L., Haggstrom, L., & Enfors, S. O. (1994). Modeling of high cell density fed batch cultivation. FEMS Microbiology Reviews, 14, 39–44.CrossRefGoogle Scholar
  4. 4.
    Bäcklund, E., Reeks, D., Markland, K., Weir, N., Bowering, L., & Larsson, G. (2008). Fed-batch design for periplasmic product retention in Escherichia coli. Journal of Biotechnology, 135, 358–365.CrossRefGoogle Scholar
  5. 5.
    Canilha, L., Carvalho, W., Felipe, M. G. A., Batista, J. A. S., & Giulietti, M. (2010). Ethanol production from sugarcane bagasse hydrolysate using Pichia stipitis. Applied Biochemistry and Biotechnology, 161, 84–92.CrossRefGoogle Scholar
  6. 6.
    Chandel, A. K., Narasu, M. L., Chandrasekhar, G., Manikyam, A., & Rao, L. V. (2009). Use of Saccharum spontaneum (wild sugarcane) as biomaterial for cell immobilization and modulated ethanol production by thermotolerant Saccharomyces cerevisiae. Biores Technol, 100, 2404–2410.CrossRefGoogle Scholar
  7. 7.
    Chandel, A. K., Singh, O. V., Rao, L. V., Chandrasekhar, G., & Narasu, M. L. (2011). Bioconversion of novel substrate Saccharum spontaneum, a weedy material, into ethanol by Pichia stipitis NCIM3498. Biores Technol, 102, 1709–1714.CrossRefGoogle Scholar
  8. 8.
    Cho, D. H., Shin, S.-J., Bae, Y., Park, C., & Kim, Y. H. (2011). Ethanol production from acid hydrolysates based on the construction and demolition wood waste using Pichia stipitis. Biores Technol, 102, 4439–4443.CrossRefGoogle Scholar
  9. 9.
    Cho, Y. H., Song, J. Y., Kim, K. M., Kim, M. K., Lee, I. Y., Kim, S. B., Kim, H. S., Han, N. S., Lee, B. H., & Kim, B. S. (2010). Production of nattokinase by batch and fed-batch culture of Bacillus subtilis. New Biotechnology, 27, 341–346.CrossRefGoogle Scholar
  10. 10.
    Chu, B. C., & Lee, H. (2007). Genetic improvement of Saccharomyces cerevisiae for xylose fermentation. Biotechnology Advances, 25, 425–441.CrossRefGoogle Scholar
  11. 11.
    Dellweg, H., Rizzi, M., Methner, H., & Debus, D. (1984). Xylose fermentation by yeasts: Comparison of Pachysolen tannophilus and Pichia stipitis. Biotechnology Letters, 6, 395–400.CrossRefGoogle Scholar
  12. 12.
    Ferreira, A. D., Mussatto, S. I., Cadete, R. M., Rosa, C. A., & Silva, S. S. (2011). Ethanol production by a new pentose-fermenting yeast strain, Scheffersomyces stipitis UFMG-IMH 43.2, isolated from the Brazilian forest. Yeast, 28, 547–554.CrossRefGoogle Scholar
  13. 13.
    Gırio, F., Fonseca, C., Carvalheiro, F., Duarte, L. C., Marques, S., & Bogel-Łukasik, R. (2010). Hemicelluloses for fuel ethanol: A review. Bioresource Technology, 101, 4775–4800.CrossRefGoogle Scholar
  14. 14.
    Gutiérrez-Rivera, B., Waliszewski-Kubiak, K., Carvajal-Zarrabal, O., & Aguilar-Uscanga, M. G. (2012). Conversion efficiency of glucose/xylose mixtures for ethanol production using Saccharomyces cerevisiae ITV01 and Pichia stipitis NRRL Y-7124. Chem Technol and Biotechnol, 87, 263–270.CrossRefGoogle Scholar
  15. 15.
    Hahn-Hägerdal, B., Karhumaa, K., Fonseca, C., Spencer-Martins, I., & Gorwa-Grauslund, M. F. (2007). Towards industrial pentose-fermenting yeast strains. Applied Microbiology and Biotechnology, 74, 937–953.CrossRefGoogle Scholar
  16. 16.
    Hong, J. (1986). Optimal substrate feeding policy for a fed batch fermentation with substrate and product inhibition kinetics. Biotechnology and Bioengineering, 28, 1421–1431.CrossRefGoogle Scholar
  17. 17.
    Huang, H., Ridgway, D., Gu, T., & Moo-Young, M. (2004). Enhanced amylase production by Bacillus subtilis using a dual exponential feeding strategy. Bioproc Biosyst Eng, 27, 63–69.CrossRefGoogle Scholar
  18. 18.
    Jeffries, T. W. (2006). Engineering yeasts for xylose metabolism. Current Opinion in Biotechnology, 17, 320–326.CrossRefGoogle Scholar
  19. 19.
    Jeffries, T. W., & Van Vleet, J. R. (2009). Pichia stipitis genomics, transcriptomics, and gene clusters. FEMS Yeast Research, 9, 793–807.CrossRefGoogle Scholar
  20. 20.
    Krahulec, S., Kratzer, R., Longus, K., & Nidetzky, B. (2012). Comparison of Scheffersomyces stipitis strains CBS 5773 and CBS 6054 with regard to their xylose metabolism: implications for xylose fermentation. Microbiologyopen, 1, 64–70.CrossRefGoogle Scholar
  21. 21.
    Kwon, E. Y., Kim, K. M., Kim, M. K., Lee, I. Y., & Kim, B. S. (2011). Production of nattokinase by high cell density fed-batch culture of Bacillus subtilis. Bioproc Biosyst Eng, 34, 789–793.CrossRefGoogle Scholar
  22. 22.
    Ladisch, M. R., & Dyck, K. (1979). Dehydration of ethanol: New approach gives positive energy balance. Science, 205, 898–900.CrossRefGoogle Scholar
  23. 23.
    Lee, J., Rodrigues, R. C., & Jeffries, T. W. (2009). Simultaneous saccharification and ethanol fermentation of oxalic acid pretreated corncob assessed with response surface. Biores Technol, 100, 6307–6311.CrossRefGoogle Scholar
  24. 24.
    Lin, T. H., Huang, C. F., Guo, G. L., Hwang, W. S., & Huang, S. L. (2012). Pilot-scale ethanol production from rice straw hydrolysates using xylose-fermenting Pichia stipitis. Biores Technol, 116, 314–319.CrossRefGoogle Scholar
  25. 25.
    Nor, Z. M., Tamer, M. I., Scharer, J. M., Moo-Young, M., & Jervis, E. J. (2001). Automated fed-batch culture of Kluyveromyces fragilis based on a novel method for on-line estimation of cell specific growth rate. Biochemical Engineering Journal, 9, 221–231.CrossRefGoogle Scholar
  26. 26.
    Pacheco Chavez, R. A., Tavares, L. C., Teixeira, A., Carvalho, J., Converti, A., & Sato, S. (2004). Influence of the nitrogen source on the productions of a-amylase and glucoamylase by a new Trichoderma sp. from soluble starch. Chem Biochem Eng, 18, 403–407.Google Scholar
  27. 27.
    Prior, B. A., Kilian, S. G., & du Preez, J. C. (1989). Fermentation of D-xylose by the yeasts Candida shehatae and Pichia stipitis. Proc Biochem, 2, 21–32.Google Scholar
  28. 28.
    Riesenberg, D. (1991). High-cell density cultivation of Escherichia coli. Current Opinion in Biotechnology, 2, 380–384.CrossRefGoogle Scholar
  29. 29.
    Scordia, D., Cosentino, S. L., Lee, J.-W., & Jeffries, T. W. (2012). Bioconversion of giant reed (Arundo donax L.) hemicellulose hydrolysate to ethanol by Scheffersomyces stipitis CBS6054. Biomass and Bioen, 39, 296–305.CrossRefGoogle Scholar
  30. 30.
    Silva, J. P., Mussatto, S. I., & Roberto, I. C. (2010). The influence of initial xylose concentration, agitation, and aeration on ethanol production by Pichia stipitis from rice straw hemicellulosic hydrolysate. Applied Biochemistry and Biotechnology, 162, 1306–1315.CrossRefGoogle Scholar
  31. 31.
    Slininger, P. J., Dien, B. S., Gorsich, S. W., & Liu, Z. L. (2006). Nitrogen source and mineral optimization enhance D-xylose conversion to ethanol by the yeast Pichia stipitis NRRL Y-7124. Applied Microbiology and Biotechnology, 72, 1285–1296.CrossRefGoogle Scholar
  32. 32.
    Slininger, P. J., Gorsich, S. W., & Liu, Z. L. (2009). Culture nutrition and physiology impact the inhibitor tolerance of the yeast Pichia stipitis NRRL Y-7124. Biotechnology and Bioengineering, 102, 778–790.CrossRefGoogle Scholar
  33. 33.
    Unrean, P., & Nguyen, N. H. (2012). Rational optimization of culture conditions for the most efficient ethanol production in Scheffersomyces stipitis using design of experiments. Biotech Prog, 28, 1119–1125.CrossRefGoogle Scholar
  34. 34.
    Unrean, P., & Nguyen, N. H. (2012). Metabolic pathway analysis of Scheffersomyces (Pichia) stipitis: Effect of oxygen availability on ethanol synthesis and flux distributions. Applied Microbiology and Biotechnology, 94, 1387–1398.CrossRefGoogle Scholar
  35. 35.
    Arslan, Y., & Eken-Saraçoğlu, N. (2010). Effects of pretreatment methods for hazelnut shell hydrolysate fermentation with Pichia stipitis to ethanol. Biores Technol, 101, 8664–8670.Google Scholar
  36. 36.
    Yong, Q., Li, X., Yuan, Y., Lai, C., Zhang, N., Chu, Q., Xu, Y., & Yu, S. (2012). An improved process of ethanol production from hemicellulose: Bioconversion of undetoxified hemicellulosic hydrolyzate from steam-exploded corn stover with a domesticated Pichia stipitis. Applied Biochemistry and Biotechnology, 167, 2330–2340.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.Biochemical Engineering and Pilot Plant Research and Development UnitKing Mongkut’s University of Technology ThonburiBangkokThailand
  2. 2.National Center for Genetic Engineering and Biotechnology (BIOTEC)PathumthaniThailand

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