Efficient Non-sterilized Fermentation of Biomass-Derived Xylose to Lactic Acid by a Thermotolerant Bacillus coagulans NL01
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Xylose is the major pentose and the second most abundant sugar in lignocellulosic feedstock. Its efficient utilization is regarded as a technical barrier to the commercial production of bulk chemicals from lignocellulosic biomass. This work aimed at evaluating the lactic acid production from the biomass-derived xylose using non-sterilized fermentation by Bacillus coagulans NL01. A maximum lactic acid concentration of about 75 g/L was achieved from xylose of 100 g/L after 72 h batch fermentation. Acetic acid and levulinic acid were identified as important inhibitors in xylose fermentation, which markedly reduced lactic acid productivity at 15 and 1.0 g/L, respectively. But low concentrations of formic acid (<2 g/L) exerted a stimulating effect on the lactic acid production. When prehydrolysate containing total 25.45 g/L monosaccharide was fermented with B. coagulans NL01, the same preference for glucose, xylose, and arabinose was observed and18.2 g/L lactic acid was obtained after 48 h fermentation. These results proved that B. coagulans NL01 was potentially well-suited for producing lactic acid from underutilized xylose-rich prehydrolysates.
KeywordsBacillus coagulans Lactic acid Xylose Non-sterilized fermentation Biomass
This study was supported by the National Natural Science Foundation of China (31070513) and Excellent Youth Foundation of Jiangsu Province of China (BK2012038). The authors are also grateful to the Major Program of the Natural Science Foundation of Jiangsu Higher Education of China (10KJA22019) and a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) for partial funding of this study.
- 1.Vijayakumar, J., Aravindan, R., & Viruthagiric, T. (2008). Recent trends in the production, purification and application of lactic acid. Chemical and Biochemical Engineering Quarterly, 22, 245–264.Google Scholar
- 8.Patel, M. A., Ou, M. S., Ingram, L. O., & Shanmugam, K. T. (2005). Simultaneous saccharification and co-fermentation of crystalline cellulose and sugar cane bagasse hemicellulose hydrolysate to lactate by a thermotolerant acidophilic Bacillus sp. Biotechnolgy Progress, 21, 1453–1460.CrossRefGoogle Scholar
- 11.Chandel, A. K., Chandrasekhar, G., Radhika, K., Ravinder, R., & Ravindra, P. (2011). Bioconversion of pentose sugars into ethanol: a review and future directions. Biotechnology and Molecular Biology Review, 6, 8–20.Google Scholar
- 20.Patel, M., Ou, M., Harbrucker, R., Aldrich, H. C., Buszko, M. L., Ingram, L. O., et al. (2006). Isolation and characterization of acid-tolerant, thermophilic bacteria for effective fermentation of biomass-derived sugars to lactic acid. Applied and Environment Microbiology, 72, 3228–3235.CrossRefGoogle Scholar
- 21.Maas, R. H. W., Bakker, R. R., Jansen, M. L. A., Visser, D., de Jong, E., Eggink, G., et al. (2008). Lactic acid production from lime-treated wheat straw by Bacillus coagulans: neutralization of acid by fed-batch addition of alkaline substrate. Applied Microbiology and Biotechnology, 78, 751–758.CrossRefGoogle Scholar
- 22.Ou, M. S., Mohammed, N., Ingram, L. O., & Shanmugam, K. T. (2009). Thermophilic Bacillus coagulans requires less cellulases for simultaneous saccharification and fermentation of cellulose to products than mesophilic microbial biocatalysts. Applied Biochemistry and Biotechnology, 155, 379–385.CrossRefGoogle Scholar
- 35.Okano, K., Yoshida, S., Yamda, R., Tanaka, T., Ogino, C., Fukuda, H., et al. (2009). Homo d-lactic acid fermentation from xylose by introduction of xylose assimilation genes and redirection of the phosphoketolase pathway to the pentose phosphate pathway in l-lactate dehydrogenase gene-deficient Lactobacillus plantarum. Applied and Environment Microbiology, 24, 7858–7861.CrossRefGoogle Scholar