Proteomic Analysis for Low and High Nitrogen-Responsive Proteins in the Leaves of Rice Genotypes Grown at Three Nitrogen Levels
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Nitrogen (N) is an essential nutrient for plants. Increase in crop production is associated with increase in N fertilizers. Excessive use of N fertilizers and the low nitrogen utilization efficiency by crop plants is a major cause for environmental damage. Therefore, to reduce the N-fertilizer pollution, there is an urgent need to improve nitrogen use efficiency. Identification and/or development of genotypes which can grow and yield well at low nitrogen levels may provide a solution. Understanding the molecular mechanism of differential nitrogen use efficiency of the genotypes may provide some clues. Keeping the above facts in mind, in this study we have identified the high N-responsive and low N-responsive contrasting rice genotypes, out of 20 genotypes that were grown at low (1 mM), moderate (10 mM), and high (25 mM) levels of N (KNO3). Proteome analysis of leaves revealed that the proteins involved in the energy production/regulation and metabolism in plant leaf tissues are differentially expressed under N treatments. Moreover, some disease-resistant and stress-induced proteins were found to be overexpressed at high levels of N. The present study could be useful in identifying proteins responding to different levels of nitrogen fertilization, which may open new avenues for a better understanding of N use efficiency, and for developing new strategies to enhance N efficiency in cereal crops.
Keywords2D Electrophoresis Mass spectrometry N-use efficiency Proteomics Rice genotypes
Conflict of Interest
The authors declare that they have no conflicts of interest.
- 3.Anjana, U. S., Iqbal, M., & Abrol, Y. P. (2007). Are nitrate concentrations in leafy vegetables within safe limits? Current Science, 92, 355–360.Google Scholar
- 5.Bates BZ, Kundzewicz SW, Palutik J (2008) eds. Observed and projected changes in climate as they relate to water. In: Climate change and water. IPCC Secretariat, Geneva.Google Scholar
- 18.Engelsberger, W. R., & Schulze, W. X. (2012). Nitrate and ammonium lead to distinct global dynamic phosphorylation patterns when resupplied to nitrogen starved Arabidopsis seedlings. The Plant Journal. doi: 10.1111/j.1365-313X.2011.04848.x.
- 19.Fan, H., Pringle, T. H., Kuhn, R. M., Karolchik, D., Diekhans, M., Haussler, D., & Kent, W. J. (2005). The UCSC Proteome Browser. Nucleic Acid Research, 33, 454–458.Google Scholar
- 20.FAO (2008) (http://faostat.fao.org/faostat).
- 23.Hakeem, K. R., Chandna, R., Ahmad, A., & Iqbal, M. (2011b). Physiological and molecular analysis of applied nitrogen in rice (Oryza sativa L.) genotypes. Rice Science, 19(1).Google Scholar
- 24.Hirel, B., & Lea, P. J. (2001). Ammonium assimilation. In P. J. Lea & J. F. Morot-Gaudry (Eds.), Plant nitrogen. Berlin: Springer.Google Scholar
- 26.Huang, Q. M., Liu, W. H., Sun, H., Deng, X., & Su, J. (2005). Agrobacterium tumefaciens mediated transgenic wheat plants with glutamine synthetases confer tolerance to herbicide. Journal of Plant Ecology, 29, 338–344.Google Scholar
- 28.Ireland, R. J., & Lea, P. J. (1999). The enzymes of glutamine, glutamate, asparagine and aspirate metabolism. In B. K. Singh (Ed.), Plant amino acids: biochemistry and biotechnology (pp. 49–109). New York: Dekker.Google Scholar
- 29.Kanwar, J. S., Katyal, J. C. (1997). Plant nutrient needs. Supply, efficiency and policy issues: 2000–2025. New Delhi: National Academy of Agricultural Sciences.Google Scholar
- 32.Ladha, J. K. (2005). Improving the recovery efficiency of fertilizer nitrogen in cereals. Journal of Indian Society of Soil Sciences, 53, 472–483.Google Scholar
- 37.Lochab, S., Pathak, R. R., & Raghuram, N. (2007). Molecular approaches for enhancement of nitrogen use efficiency in plants. In Y. P. Abrol, N. Raghuram, & M. S. Sachdev (Eds.), Agricultural nitrogen use and its environmental implications (pp. 327–350). New Delhi: IKI.Google Scholar
- 42.Mengel, K., & Kirby, E. A. (1978). Potassium. In K. Mengel & E. A. Kirby (Eds.), Principles of plant nutrition (pp. 376–390). Switzerland: International Potash Institute.Google Scholar
- 49.Prasad, R. (1998). Fertilizer urea, food security and the environment. Current Science, 75, 677–683.Google Scholar
- 54.Shigeto, J., Yoshihara, S., Adam Suaad, E. H., Sueyoshi, K., Sakamoto, A., Morikawa, H., & Takahashi, M. (2006). Genetic engineering of nitrite reductase gene improves uptake and assimilation of nitrogen dioxide by Rhaphiolepis umbellate (Thunb.) Makino. Plant Biotechnology, 23, 111–116.CrossRefGoogle Scholar
- 56.Thiellement, H., Plomion, C., & Zivy, M. (2001). Proteomics from protein sequence to function. Oxford: BIOS Scientific.Google Scholar
- 58.Wang, R., Guegler, K., Labrie, S. T., & Crawford, N. M. (2000). Genomic analysis of a nutrient response in Arabidopsis reveals diverse expression patterns and novel metabolic and potential regulatory genes induced by nitrate. The Plant Cell, 12, 1491–1509.Google Scholar