Applied Biochemistry and Biotechnology

, Volume 167, Issue 8, pp 2131–2143 | Cite as

Effects of Ionic Liquids on the Growth of Arthrobacter simplex and Improved Biodehydrogenation in an Ionic Liquid-Containing System with Immobilized Cells

  • Jin Huang
  • Songlin Xie
  • Junyao He
  • Pu WangEmail author


Dehydrogenated derivatives of corticosteroids are usually more effective than their precursors in treating diseases. In this study, the toxicity of seven water-miscible ionic liquid and three organic solvents to the biocatalyst Arthrobacter simplex UR016 was tested to evaluate the possibility of biodehydrogenation from 17α-hydroxy-16β-methyl-pregna-4,9(11)-diene-3,20-dione (HMPDD) to 17α-hydroxy-16β-methyl-pregna-1,4,9(11)-triene-3,20-dione (HMPTD) in an ionic liquid-containing system. Although most tested room temperature ionic liquids (RTILs) showed higher toxicities to A. simplex UR016 than organic solvents, bacterial growth was promoted in the presence of [EMIM](l)-Lac or [BMIM](l)-Lac at concentrations below 2.5 mmol/l, especially [EMIM](l)-Lac, presented the lowest toxicity to A. simplex. Following immobilization investigations, a conversion ratio of 89.9 % was achieved with a cell biomass of 10 g/l (dry cell weight/reaction mixture volume) in the polyethylene glycol (PEG)-modified calcium alginate gel bead, a suitable matrix for cell immobilization. Further studies indicated that the conversion ratio can be improved by increasing cell loading to 60 beads per flask (containing 30 ml reaction mixture). Under optimal conditions with a [EMIM](l)-Lac content of 0.3 %, the conversion ratio reached 93.4 %, the highest value ever reported.


Arthrobacter simplex Ionic liquids Biodehydrogenation Immobilized cells Growth 



This work was financially supported by grants from the National Natural Science Foundation of China (21076193) and Zhejiang Provincial Natural Science Foundation of China (Y407289).


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.College of Pharmaceutical ScienceZhejiang University of TechnologyHangzhouChina
  2. 2.Zhejiang Pharmaceutical CollegeNingboChina

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