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Applied Biochemistry and Biotechnology

, Volume 167, Issue 3, pp 536–551 | Cite as

Mushroom Polysaccharides and Lipids Synthesized in Liquid Agitated and Static Cultures. Part I: Screening Various Mushroom Species

  • Panagiota Diamantopoulou
  • Seraphim Papanikolaou
  • Maria Kapoti
  • Michael Komaitis
  • George Aggelis
  • Antonios PhilippoussisEmail author
Article

Abstract

The effect of four synthetic media containing glucose (initial concentration 30 g l−1) on mycelial growth, exopolysaccharides (EPS) and cellular lipids production was examined in 11 mushroom species after 12 and 16 days of culture in static- and shake-flasks. Fatty acid analysis of cellular lipids produced was also performed. Agitation had a positive effect on biomass production, glucose consumption and lipid biosynthesis. Media that favoured the production of biomass were not suitable for EPS biosynthesis and vice versa. Biomass values varied from ∼1.0 g l−1 (Lentinula edodes) to ∼19 g l−1 (Pleurotus ostreatus), while the highest EPS quantity achieved ranged between 1.6 and 1.8 g l−1 (for Ganoderma lucidum and L. edodes, respectively). Quantities of total cellular lipids varied between 2.5 and 18.5 % w/w, in dry mycelial mass for the fungi tested. Lipid in dry weight values were influenced by the medium composition. Cellular lipids presented noticeable quantities of poly-unsaturated fatty acids like linoleic acid. Compared to most of the mushrooms tested, lipids of Volvariella volvacea were more saturated. The ability of several mushroom species of our study to produce in notable quantities the above-mentioned added-value compounds renders these fungi worthy for further investigations.

Keywords

Ascomycetous Basidiomycetous Biomass Cellular lipids Exopolysaccharides Submerged cultures 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Panagiota Diamantopoulou
    • 1
  • Seraphim Papanikolaou
    • 2
  • Maria Kapoti
    • 1
  • Michael Komaitis
    • 2
  • George Aggelis
    • 3
  • Antonios Philippoussis
    • 1
    Email author
  1. 1.Laboratory of Edible Fungi, Institute of Technology of Agricultural ProductsNational Agricultural Research Foundation (NAGREF)AthensGreece
  2. 2.Department of Food Science and TechnologyAgricultural University of AthensAthensGreece
  3. 3.Unit of Microbiology, Division of Genetics, Cell and Development Biology, Department of BiologyUniversity of PatrasPatrasGreece

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