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Applied Biochemistry and Biotechnology

, Volume 167, Issue 4, pp 791–808 | Cite as

Nano-TiO2-Induced Apoptosis by Oxidative Stress-Mediated DNA Damage and Activation of p53 in Human Embryonic Kidney Cells

  • Ramovatar Meena
  • Madhu Rani
  • Ruchita Pal
  • Paulraj RajamaniEmail author
Article

Abstract

The aim of the present study is to explore the mechanism of cytotoxic and genotoxic effects of TiO2 nanoparticles on human embryonic kidney (HEK-293) cells. Toxicity was evaluated using changes in various cellular parameters of HEK-293 cells like morphology, viability, metabolic activity, oxidative stress and apoptosis. Oxidative stress was measured by the level of reactive oxygen species (ROS), lipid peroxidation, superoxide dismutase, catalase and glutathione peroxidase. Apoptosis induced by nano-TiO2 was characterized by PI staining and DNA ladder assay. Furthermore, apoptotic proteins such as p53 and Bax were analysed by western blot. Our results indicate that nano-TiO2 induces cytotoxicity in a time- and dose-dependent manner. Oxidative stress and apoptosis were induced by exposure to nano-TiO2. Moreover, the expression of p53, Bax and caspase-3 were increased in a dose-dependent pattern. In conclusion, ROS-mediated oxidative stress, the activation of p53, Bax, caspase-3 and oxidative DNA damage are involved in the mechanistic pathways of nano-TiO2-induced apoptosis in HEK-293 cells.

Keywords

Nano-TiO2 Cytotoxicity Oxidative stress p53 Apoptosis 

Notes

Acknowledgments

The authors are thankful to Advance Instrumentation Research Facility (AIRF), JNU New Delhi, for confocal microscopy, transmission electron microscopy, scanning electron microscopy and EDXRF instruments.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Ramovatar Meena
    • 1
  • Madhu Rani
    • 2
  • Ruchita Pal
    • 3
  • Paulraj Rajamani
    • 1
    Email author
  1. 1.School of Environmental SciencesJawaharlal Nehru UniversityNew DelhiIndia
  2. 2.School of BiotechnologyJawaharlal Nehru UniversityNew DelhiIndia
  3. 3.Advanced Instrumentation Research FacilityJawaharlal Nehru UniversityNew DelhiIndia

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